Journal of South China University of Technology(Natural Science Edition) ›› 2004, Vol. 32 ›› Issue (10): 76-79.

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Cloning and Homology Analysis of the Conservative Sequence of Phytoene Synthase Gene from Dunaliella Salina

Jiang Jian- guo1 Yan Yuan1 Song Dong- lin2   

  • Received:2003-09-25 Online:2004-10-20 Published:2015-09-08
  • Contact: 姜建国（1964－），男，汉族，博士，副研究员，主要从事海洋天然产物方面的研究．
  • About author:姜建国（1964－），男，汉族，博士，副研究员，主要从事海洋天然产物方面的研究．

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Abstract: In this paper, according to the cDNA conservative sequence of Psy from two species of microalgae (Dunaliella bardawil and Haematococcus pluvialis) and the amino acid sequences of Psy from six species of plants (Arabidopsis thaliana, Oryza sative, Tagetes erecta, Lycopersicon esculentum, Capsicum annuum and Daucus carota) , the forward primer, namely, 5'-ACGCCAAGACCTTCTA-3', and the reverse primer, namely, 5'-GTTGTCATAGTCATTCTTCTC-3' were designed. The amplification by using D.salina genomic DNA as the temple was then performed with an annealing temperature reduction of 0.5 ℃ per cycle in 46～35 ℃, thus obtaining a amplified fragments of about (2 490 bp, ) which was finally identified as partial fragment of the Psy gene from D.salina by the homology analysis of NCBI/BlastP.

Key words: Dunaliella salina, phytoene synthase, conservative sequence, homology analysis