Screening of Cellulase-producing Strains and Growth Conditions of Enzyme

Journal of South China University of Technology(Natural Science Edition) ›› 2003, Vol. 31 ›› Issue (3): 45-48.

• Food Science & Technology • Previous Articles Next Articles

Screening of Cellulase-producing Strains and Growth Conditions of Enzyme

Li Hai-bin¹ Li Lin¹ Huang Ri-bo² Guo Si-yuan¹ Cai Miao-yan¹

1．College of Food＆Biological EngineeringSouth China Univ．of Tech．Guangzhou510640China；
2．Experiment Centre of BiotechnologyGuangxi UniversityNanning530004China

Online:2003-03-20 Published:2022-04-22
Contact: 黎海彬（1964－）男博士生任职于广西大学主要从事生物工程及其分离纯化技术的研究．
About author:黎海彬（1964－）男博士生任职于广西大学主要从事生物工程及其分离纯化技术的研究．

Abstract

Abstract: Thermomonospora fusca was chosen as an original strain for screening high yield cellulase mutants．Spora suspension of the organism was treatment with UV for60s and80s alternately．A high cellulase-producing mutant strain PE-211was obtained by screening using the methods of transparent zone and shaking culture．The activity of mutant strains PE-211was enhanced for more than one time comparing with that of the original strain．The CMCase-producing conditions of PE-211were studied too．

Key words: Thermomonospora fusca, mutation, cellulase

CLC Number:

Li Hai-bin, Li Lin, Huang Ri-bo, et al. Screening of Cellulase-producing Strains and Growth Conditions of Enzyme[J]. Journal of South China University of Technology(Natural Science Edition), 2003, 31(3): 45-48.

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Screening of Cellulase-producing Strains and Growth Conditions of Enzyme

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