Journal of South China University of Technology (Natural Science Edition) ›› 2014, Vol. 42 ›› Issue (3): 137-144.doi: 10.3969/j.issn.1000-565X.2014.03.022

• Food Science & Technology • Previous Articles    

Identification of Lactobacillus sp．DMDL 9010 by Upstream and Downstream DNA Sequences of L- Lactate Dehydrogenase 1

Liu Dong- mei Fei Yong- tao Wang Pan Chen Gu Xiao Xing- long Wu Hui Tang Yu- qian   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2013-11-14 Online:2014-03-25 Published:2014-02-19
  • Contact: 刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物的利用与控制研究． E-mail:liudm@scut.edu.cn
  • About author:刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物的利用与控制研究．
  • Supported by:

    国家自然科学基金资助项目(31101254);广东省自然科学基金资助项目(S2011010005679)

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Abstract:

Lactobacillus sp.DMDL 9010 was isolated from fermented vegetables and was used for the degradation ofnitrites in MRS broth,and the results are compared with those of other four lactic acid bacteria including LCR 719,LB 1.83,ST 1.204 and LP 8140,with the best nitrite degradation effect of DMDL 9010 after 24- h fermentation(P≤0.001) being revealed. Then,the identification scope of DMDL 9010 was reduced to Lactobacillus plantarumand Lactobacillus pentosus with the help of 16S rDNA sequence,and the strain was identified as L.pentosus by tes-ting physiological and biochemical characters,although no specific stripe was obtained in the subsequent PCR.More-over,a homologous DNA sequence of L- lactate dehydrogenase 1 (ldhL1) with a similarity of 90% was found in thegenome of L.pentosus and L.plantarum,and the low DNA sequence similarity in the upstream and downstreamfragments of ldhL1 could be regarded as an identification criterion of the two strains.Finally,after the PCR amplifi-cation and sequencing of the upstream and downstream fragments of ldhL1,the above- mentioned sequences werecompared via the bioinformatics analysis,and DMDL 9010 was identified as L.plantarum.

Key words: bacterium, identification, DNA sequence, fermentation