Journal of South China University of Technology (Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (9): 28-33.doi: 10.3969/j.issn.1000-565X.2011.09.006

• Food Science & Technology • Previous Articles     Next Articles

Optimization of Homogenate Extraction Process of Limonin via Statistical Methods

Liu Jing  Liu Can  Rong Yong-hai  Rong Long   

  1. School of Biological Science and Medical Engineering,Beihang University,Beijing 100191,China
  • Received:2011-03-09 Revised:2011-05-12 Online:2011-09-25 Published:2011-08-02
  • Contact: 荣龙(1964-) ，男，教授，博士生导师，主要从事生物资源综合利用研究． E-mail: ronglong@ buaa．edu．cn E-mail:liujing@ be.buaa.edu.cn
  • About author:刘静(1983-) ，女，博士生，主要从事食品生物技术研究．
  • Supported by:

    广东省教育部产学研结合项目( 2009B090300323)

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Abstract:

The homogenate extraction of limonin respectively by means of the orthogonal design,the uniform design and the response surface methodology ( RSM) ,with the limonin yield,as the evaluation index. Then,by combining the optimal process with the method of crystallization,limonin was extracted from the seeds of oranges ( Citrus reticulata Blanco) in Guangzhou. Moreover,its purity was analyzed through the high-performance liquid chromatography. The results show that the purity of the sample reaches 95%,that the effects of the three statistical methods on the yield are close to each other,and that,by means of RSM,the maximum limonin yield in up to( 6. 65 ± 0. 01) mg /g in the optimal conditions,namely,an ethanol concentration of 71. 7% ( volume fraction) ,a solvent-solid ratio of 28. 8mL /g and a rotating speed of 3953 r /min.

Key words: limonin, homogenate extraction, orthogonal test design, uniform design, response surface methodology