Journal of South China University of Technology (Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (5): 138-142.doi: 10.3969/j.issn.1000-565X.2011.05.024

• Biological Engineering • Previous Articles     Next Articles

Transformation of Polygonum cuspidatum Resveratrol Synthase Gene into Arabidopsis thaliana

Liu Zhong-yu1  Zhuang Chu-xiong2  Sheng Shu-jing1  Shao Li1  Zhao Shu-jin3   

  1. 1．School of Biological Science and Engineering，South China University of Technology，Guangzhou 510006，Guangdong，China；2．College of Life Science，South China Agricultural University，Guangzhou 510642，Guangdong，China；3．General Hospital of Guangzhou Military Command，Guangzhou 510010，Guangdong，China
  • Received:2010-10-12 Revised:2010-12-29 Online:2011-05-25 Published:2011-04-01
  • Contact: 赵树进(1958-)，男，教授，博士生导师，主要从事生物制药研究 E-mail:gzzsjzhs@163.com
  • About author:柳忠玉(1978-)，女，博士生，主要从事药用植物次生代谢研究
  • Supported by:

    广东省自然科学基金资助项目(10151001002000012)；广东省科技计划项目(2010B060200009

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Abstract:

In order to reveal the effectiveness of Polygonum cuspidatum resveratrol synthase（PcRS） gene in improving the resveratrol accumulation in foreign species,first,the plant expression vector pCAM1380-35S-PcRS was constructed and introduced into Arabidopsis thaliana by Agrobacterium tumefaciens by means of the floral dip me-thod.Next,the transformants were screened on the medium containing hygromycin.Then,the integration of PcRS gene into the transgenic plants was verified via the PCR and the Southern blotting.Moreover,independent homozygous transgenic Arabidopsis thaliana lines with genetical stability were obtained after the selection of T3 progenies,and the expression of the gene transferred into Arabidopsis thaliana was confirmed by Northern blotting.Finally,an end product,namely trans-piceid,was identified by HPLC,the content of which in 2-week-old plants being 136 μg/g（fresh weight） or 1 813 μg/g（dry weight）.

Key words: Polygonum cuspidatum, resveratrol synthase gene, Arabidopsis, resveratrol, piceid