华南理工大学学报(自然科学版) ›› 2010, Vol. 38 ›› Issue (8): 100-104.doi: 10.3969/j.issn.1000-565X.2010.08.019

• 食品科学与技术 • 上一篇    下一篇

蛋清抗氧化肽增效剂的优化

林松毅1  郭洋1  王莹2  刘静波1  郑淋1  殷涌光2   

  1. 1.吉林大学 军需科技学院, 吉林 长春 130062; 2.吉林大学 生物与农业工程学院, 吉林 长春 130025
  • 收稿日期:2010-02-04 修回日期:2010-03-23 出版日期:2010-08-25 发布日期:2010-08-25
  • 通信作者: 刘静波(1962-),女,博士,教授,主要从事营养与功能食品研究.E-mail:ljb168@sohu.com E-mail:linsongyi730@163.com
  • 作者简介:林松毅(1970-),女,博士,副教授,主要从事功能食品研究与开发.
  • 基金资助:

    国家“863”计划项目(2007AA10Z329);中央高校基本科研业务费科学前沿与交叉学科创新项目(200810029)

Optimization of Synergist of Antioxidant Peptide Derived from Egg White Protein

Lin Song-yi Guo Yang1  Wang Ying2  Liu Jing-bo1  Zheng Lin1  Yin Yong-guang2   

  1. 1. College of Quartermaster Technology, Jilin University, Changchun 130062, Jilin, China; 2. College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, Jilin, China
  • Received:2010-02-04 Revised:2010-03-23 Online:2010-08-25 Published:2010-08-25
  • Contact: 刘静波(1962-),女,博士,教授,主要从事营养与功能食品研究.E-mail:ljb168@sohu.com E-mail:linsongyi730@163.com
  • About author:林松毅(1970-),女,博士,副教授,主要从事功能食品研究与开发.
  • Supported by:

    国家“863”计划项目(2007AA10Z329);中央高校基本科研业务费科学前沿与交叉学科创新项目(200810029)

摘要: 以DPPH(1,1-二苯基-2-苦肼基)清除率为评价指标,考察了NaCl、糖类、柠檬酸单独添加以及两两或三者合用对蛋清抗氧化肽活性的影响,并在此基础上运用响应面分析法,优化出增效剂的最佳组合.结果表明,NaCl、麦芽糖及柠檬酸均能显著提高蛋清抗氧化肽的活性,可作为蛋清抗氧化肽的抗氧化增效剂单剂,三者合用时增效效果最佳,最佳组合为NaCl1.4%、麦芽糖3.0%、柠檬酸0.24%,在此条件下蛋清抗氧化肽对DPPH的IC50值(DPPH清除率达到50%时蛋清抗氧化肽的质量浓度)为0.27mg/mL,增效比可达3.96.

关键词: 蛋清蛋白, 抗氧化性, 响应面法, 增效剂, 优化

Abstract:

This paper focuses on the effects of single or complex addition of NaCl, carbohydrates, citric acid on the antioxidant activity of the peptide derived from egg white protein, with the DPPH scavenging activity as the evaluation index. Moreover, the best combination of synergist is optimized via the response surface methodology. The results show that ( 1 ) NaCl, maltose and citric acid all signifieantly improve the antioxidant activity of the peptide derived from egg white protein, so that they can be used as an antioxidant synergist independently; (2) the triadic combination of NaCl, maltose and citric acid is of the best enhancement effect ; and (3) in the optimal combination ( i. e. , 1.4% NaCl, 3.0% maltose and 0. 24% citric acid), the synergist may result in a IC50 value on DPPH radicals of 0.27 mg/mL and a synergism value being up to 3.96.

Key words: egg white protein, antioxidant property, response surface methodology, synergist, optimization