关键词: 顺 1, 2-二氯乙烯;好氧矿化, 16S rRNA;群落结构, Delftia 菌属

Abstract: Organic chlorine solvent，as an important chemical raw material，may lead to serious pollution in the process of use． At present，the concentration of cis 1，2-dichloroethylene (cDCE) has been detected in ground- water of many organic chlorine contaminated sites，and its toxicity is much greater than that of trichloroethylene and tetrachloroethylene． In view of the problem of microbial aerobic mineralization of cDCE in groundwater contamina- ted by organochlorine，organic chlorine contaminated groundwater was taken as a source of bacteria in a certain area． After 338 days' enrichment and domestication，the cDCE mineralized aerobic bacteria was finally cultured． The cultured cDCE aerobic mineralized mixed bacteria showed better ability to metabolize cDCE． At the initial concen- tration of cDCE concentration of 1 mg/L，the degradation rate was 38. 1% after 16 days． In order to enrich its bio- logical information，16S rRNA high-throughput metagenomic sequencing technology was used to analyze mixed bac- teria in different periods during domestication，and molecular biological information of mixed bacteria was ob- tained． The samples in different culture stages have certain continuity; their diversity decreases first and then in- crease with the culture of microorganisms． At the same time，the community structure of microorganisms at diffe- rent stages was obtained． Combined with other biological information and the ability of microorganisms to metabo- lize cDCE during culture，it is speculated that cDCE mineralized aerobic bacteria belongs to the genus Delftia．

Key words: cis 1, 2-dichloroethylene, aerobic mineralization, 16S rRNA, community structure, Delftia