华南理工大学学报(自然科学版) ›› 2014, Vol. 42 ›› Issue (7): 124-131.doi: 10.3969/j.issn.1000-565X.2014.07.020

• 化学化工 • 上一篇    下一篇

蔗渣乙醇预处理及其对酶解的影响

武书彬 徐绍华   

  1. 华南理工大学 制浆造纸工程国家重点实验室,广东 广州 510640
  • 收稿日期:2014-03-21 修回日期:2014-05-06 出版日期:2014-07-25 发布日期:2014-06-01
  • 通信作者: 武书彬(1965-),男,教授,博士生导师,主要从事植物纤维化学与化工、二次纤维清洁高效利用新技术等研究. E-mail:shubinwu@scut.edu.cn
  • 作者简介:武书彬(1965-),男,教授,博士生导师,主要从事植物纤维化学与化工、二次纤维清洁高效利用新技术等研究.
  • 基金资助:

    国家“863”计划项目( 2012AA101806) ; 国家自然科学基金资助项目( 31270635) ; 华南理工大学中央高校基本科研业务费专项资金资助项目( 2014ZP0014)

Ethanol Pretreatment of Sugarcane Bagasse and Its Effect on Enzymatic Hydrolysis

Wu Shu-bin Xu Shao-hua   

  1. State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2014-03-21 Revised:2014-05-06 Online:2014-07-25 Published:2014-06-01
  • Contact: 武书彬(1965-),男,教授,博士生导师,主要从事植物纤维化学与化工、二次纤维清洁高效利用新技术等研究. E-mail:shubinwu@scut.edu.cn
  • About author:武书彬(1965-),男,教授,博士生导师,主要从事植物纤维化学与化工、二次纤维清洁高效利用新技术等研究.
  • Supported by:

    国家“863”计划项目( 2012AA101806) ; 国家自然科学基金资助项目( 31270635) ; 华南理工大学中央高校基本科研业务费专项资金资助项目( 2014ZP0014)

摘要: 采用自催化乙醇法对蔗渣原料进行预处理,并通过傅里叶变换红外光谱、扫描电镜、X 射线衍射仪对预处理样品进行分析,然后进行酶解. 结果表明: 蔗渣原料经乙醇预处理后,有大量木质素溶出和半纤维素水解; 预处理样品的相对结晶度提高了32.97%; 蔗渣纤维表面碎化,细小纤维暴露出来,极大地提高了酶解效率; 当温度为195℃、乙醇体积分数为40%、保温时间为30min 时,蔗渣原料木素去除率为57.97%,此时100 g 蔗渣原料经此预处理所得预处理液中的木糖量为8.59 g,占原料中总木糖的35.16%,预处理样经酶解,所得酶解液中葡萄糖含量为40.29 g,占原料中总葡萄糖的92.15%; 初步实现了蔗渣原料中半纤维素和纤维素的逐步分离,同时得到大量乙醇木素; 最优酶用量为10 FPU/g( 以每克固体浆料计) ,固液比为1 ∶ 40( g /mL) .

关键词: 蔗渣, 乙醇, 酶解, 脱木素

Abstract:

Sugarcane bagasse was pretreated via the self-catalyzed ethanol delignification,and the pretreated sampleswere analyzed by means of FT-IR,SEM and XRD,and then the corresponding enzymatic hydrolysis wasmade.The results show that ( 1) the pretreatment makes some lignin and hemicellulose hydrolyzed and the relativecrystallinity of pretreated samples increases by 32.97%; ( 2) after the treatment,the surface of bagasse fibres isfragmented and fine fibres are exposed with a result that the efficiency of enzymatic hydrolysis is greatly improved;( 3) when the pretreatment is performed at the temperature of 195℃ with a volume fraction of ethanol being 40%and warmth remaining for 30 min,57.97% of lignin in the solid material are removed,and 8.59 g xylose isobtained from pre-hydrolyzate,which accounts for 35.16% of total xylose in the raw bagasse of 100 g; ( 4) duringthe subsequent enzymatic hydrolysis,40.29 g glucose is obtained,which accounts for 92.15% of total glucose inthe raw bagasse; ( 5) the gradual separation of hemicellulose and cellulose in raw material is preliminarily achievedand at the same time a large amount of ethanol lignin is obtained; and ( 6) the optimal enzyme dosage is 10 FPU/gand the solid-to-liquid ratio ( dry substrate) is 1∶ 40( g /mL) .

Key words: sugarcane bagasse, ethanol, enzymatic hydrolysis, delignification