华南理工大学学报(自然科学版) ›› 2007, Vol. 35 ›› Issue (4): 42-45.

• 环境科学与技术 • 上一篇    下一篇

仙人掌多糖的超滤膜分离提取及其影响因素

杨宁1 赵谋明1† 崔春1 张桂和1 杨宝2   

  1. 1.华南理工大学 轻工与食品学院,广东 广州 510640; 2. 中国科学院 华南植物园,广东 广州 510650
  • 收稿日期:2006-09-27 出版日期:2007-04-25 发布日期:2007-04-25
  • 通信作者: 赵谋明,教授,博士生导师. E-mail: femmzhao@scut. edu. cn E-mail:yning789@126.com
  • 作者简介:杨宁(1964-),男,博士生,高级工程师,主要从事食品生物技术研究.
  • 基金资助:

    广东省科技计划项目(2005B20401024) ;广州市科技攻关项目(2004Z1-E0061 )

Extraction of Polysaccharides from Opuntia monacantha Haw. by Membrane Ultrafiltration and Corresponding Inf1uence Factors

Yang Ning1  Zhao Mou-ming1 Cui ChunZhang Gui-heyang Bao2   

  1. 1. School of Light Industry and Food Sciences , South China Univ. of Tech. , Guangzhou 510640 , Guangdong , China;2. South China Botanical Garden , Chinese Academy of Sciences , Guangzhou 510650 , Guangdong , China
  • Received:2006-09-27 Online:2007-04-25 Published:2007-04-25
  • Contact: 赵谋明,教授,博士生导师. E-mail: femmzhao@scut. edu. cn E-mail:yning789@126.com
  • About author:杨宁(1964-),男,博士生,高级工程师,主要从事食品生物技术研究.
  • Supported by:

    广东省科技计划项目(2005B20401024) ;广州市科技攻关项目(2004Z1-E0061 )

摘要: 应用不同截留分子质量的超滤膜对仙人掌多糖浸提液进行分离、纯化和浓缩,并分析了超滤压力、超滤温度、纯化时间、料液体和、流量、浓缩倍数等对超滤过程的影响,得出了最适宜的操作条件.研究结果表明,当选用截留分子质量为10000ku 的超滤膜、超滤压力为0.20MPa 、超滤温度为25℃、料液体积流量控制在0.04 -0.05νmm 、浓缩倍数为5 -7 倍时,粗多糖得率达0.95%. 与传统的真空浓缩法相比,超滤浓缩法的粗多糖得率较高,所得多糖的品质也得到了改善,减轻了后续工艺进一步分离纯化多糖的负担.

关键词: 超滤, 膜分离, 浓缩, 纯化, 仙人掌多糖

Abstract:

The separation , purification and concentration of the polysaccharides from Opuntia monαcαnthαHaw.were carried out via membrane ultrafitration using the membranes with different cut-off molecular masses. The effects of several parameters such as the pressure , the temperature , the purification time , the flow rate and the con-centration multiple on the ultrafiltration process were then investigated. The optimal conditions for the ultrafiltration were finally determined. It is found that , for the membrane with a cut-off molecular mass of 10 000 ku , when the pressure , the temperature , the flow rate and the concentration multiple are respectively0. 20 MPa , 25℃ , 0. 04-0.05 Umin and 5 -7 , a yield of crude polysaccharides of 0.95%can be reached. Moreover , as compared with the traditional vacuum concentration , membrane ultrafiltration is easier to obtain high-quality crude polysaccharides with a higher yield , which is helpful to the further extraction and purification of polysaccharides in the subsequent process.

Key words: ultrafiltration, membrane separation, concentration, purification, cactus polysaccharide