华南理工大学学报(自然科学版) ›› 2007, Vol. 35 ›› Issue (12): 121-125.

• 食品科学与技术 • 上一篇    下一篇

超声处理对酵母细胞的致死及相关影响

周丽珍 李冰 李琳 张喜梅   

  1. 华南理工大学 轻工与食品学院,广东 广州 510640
  • 收稿日期:2007-03-20 出版日期:2007-12-25 发布日期:2007-12-25
  • 通信作者: 周丽珍(1977-) ,女,博士生,主要从事糖类物质及其药物的制备与生物利用、食品生物技术研究. E-mail:gzzhoulizhen@163.com
  • 作者简介:周丽珍(1977-) ,女,博士生,主要从事糖类物质及其药物的制备与生物利用、食品生物技术研究.
  • 基金资助:

    国家自然科学基金重点项目( 20436020) ;广东省自然科学基金重点项目(04105934) ;广东省博士启动基金项目(05300 l7 5 )

Inactivation of Yeast Cells by Ultrasound Disinfection and Related Influences

Zhou Li-zhen  Li Bing  Li Lin  Zhang Xi-mei   

  1. School of Light Industlγand Food Sciences , South China Univ. of Tech. , Guangzhou 510640 , Guangdong , China
  • Received:2007-03-20 Online:2007-12-25 Published:2007-12-25
  • Contact: 周丽珍(1977-) ,女,博士生,主要从事糖类物质及其药物的制备与生物利用、食品生物技术研究. E-mail:gzzhoulizhen@163.com
  • About author:周丽珍(1977-) ,女,博士生,主要从事糖类物质及其药物的制备与生物利用、食品生物技术研究.
  • Supported by:

    国家自然科学基金重点项目( 20436020) ;广东省自然科学基金重点项目(04105934) ;广东省博士启动基金项目(05300 l7 5 )

摘要: 超声处理作为新兴的非热杀菌技术,由于对超声处理条件的作用规律和对微生物细胞的影响研究不足,在实际应用中还不成熟.文中以酿酒酵母为对象,考察超声杀茵处理效果的三个主要影响因素(即超声功率、时间和脉冲占空比)对细胞致死作用的影响规律,并采用选择性平板法对超声引起的细胞亚致死效应进行检测,采用TEM 对细胞超微结构变化进行检测.结果表明:超声功率及时间对细胞的致死作用影响显著;超声处理后细胞未检出显著的亚致死损伤;超声处理对细胞的超微结构破坏大,表明结构完整性的破坏可能是细胞死亡的直接原因.

关键词: 超声, 杀菌, 致死, 酵母, 细胞

Abstract:

As an emerging nonthermal disinfection technique , ultrasonic treatment is still in its infancy of industrial application due to the insufficient investigations into its inf1uencing rules on disinfection and its effects on cells. In order to solve this prohlem , Saccharomyces cerevisiae was chosen as the ohject to reveal the effects of three ultrasonic parameters on the inactivation of yeast cells. The parameters included the ulrasonic power , the irradiation time and the pulse ratio. Moreover , the selective medium method and the TEM were adopted to investigate the cell sublethal injmγcaused hy ultrasonic treatment and the cell ultramicrostructure after the treatment , respectively. The results show that (1) hoth the ultrasonic power and the irradiation time have significant positive effects on the inactivation of cells j (2) no s山letl叫injured cells are detected after the ultrasonic treatment j and (3) ultrasonic irradiation may result in great damage to cell ultramicrostructure. It is thus concluded that the damage of structural integrity could he the direct reason for the death of cells.

Key words: ultrasonic, disinfection, inactivation, yeast, cell