华南理工大学学报(自然科学版) ›› 2005, Vol. 33 ›› Issue (3): 87-90.

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九孔鲍肠道及水体中产几丁质酶菌群的测定

王志 蔡俊鹏 温其标   

  1. 华南理工大学 轻工与食品学院,广东 广州 510640
  • 收稿日期:2004-03-23 出版日期:2005-03-25 发布日期:2005-03-25
  • 通信作者: 王志(1977-),男,博士生,主要从事食品生物技术的研究 E-mail:Wangzhi2000@vip.sina.com
  • 作者简介:王志(1977-),男,博士生,主要从事食品生物技术的研究
  • 基金资助:

    广东省自然科学基金重点资助项目(020964);华南理工大学高水平大学建设苗子项目(321-D76020)

Mensuration of Chitinase-Producing Abilities of Strains Isolated From Digestion Guts and Farming Water of Hafiotis Diversicolor Supertexta

Wang Zhi  Cai Jun-peng  Wen Qi-biao   

  1. College of Light Chemistry and Food Science,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China
  • Received:2004-03-23 Online:2005-03-25 Published:2005-03-25
  • Contact: 王志(1977-),男,博士生,主要从事食品生物技术的研究 E-mail:Wangzhi2000@vip.sina.com
  • About author:王志(1977-),男,博士生,主要从事食品生物技术的研究
  • Supported by:

    广东省自然科学基金重点资助项目(020964);华南理工大学高水平大学建设苗子项目(321-D76020)

摘要: 通过特定的TCBS(Thiosufate-Citrate.Bile Salts—Sucrose)和筛选培养基,对九孔鲍肠道及其养殖水体中菌群的产几丁质酶能力进行了研究.结果表明,在鲍鱼肠道及其养殖水体中均存在有能分泌几丁质酶的微生物.在鲍鱼肠道中,共分离到12株菌株,占总细菌的34.3% ,它们分另q属于V/briofluvialis和Vibrio minicus;在鲍鱼养殖水体中则分离到4株菌株,占总细茵的11.8%,属于Vibriofluvialis.比较鲍鱼肠道和养殖水体中产几丁质酶菌株的比例及其能力的大小,可以发现前者的比例高于后者,由此表明了鲍鱼肠道对菌株的选择性.

关键词: 九孔鲍, 肠道, 养殖水体, 细菌, 几丁质酶

Abstract:

The chitinase-producing abilities of strains isolated from both farming water and digestion guts of Haliotis diversicolor supertexta were studied by a special TCBS(Thiosufate-Citrate-Bile Salts-Sucrose)and by screening the culture media.The resuhs indicate that there are chitinase-producing strains in farming water and digestion gu ts of the abalone.12 bacteria were isolated from abalone digestion gu ts.which account for 34.3%of the total bacterial populations in the guts.Species identification shows that these chitinase-producing strains belong to Vibrio fluvialis and Vibrio minicus.From the ab alone farming water,4 chitinase-producing strains were isolated,which account for 1 1.8% of the total bacterial populations.These 4 strains belong to Vibrio fluvialis.Compared with the farming wa-ter,it is clear that there are more chitinase-producing strains in abalone digestion gu ts,and abalone digestion gu ts display selectivity toward the chitinase-producing strains.

Key words: Haliot&, diversicolor supertexta, digestion gu ts, farm ing water, bacteria, chitinase