降解聚乙烯醇菌株原生质体的制备及融合

华南理工大学学报(自然科学版) ›› 2004, Vol. 32 ›› Issue (5): 74-79.

降解聚乙烯醇菌株原生质体的制备及融合

廖劲松¹ 郭勇¹ 庄桂²

1．华南理工大学食品与生物工程学院广东广州510640；2．郑州工程学院生物工程系河南郑州450052

收稿日期:2003-11-12 出版日期:2004-05-20 发布日期:2015-09-08
通信作者: 廖劲松（1976－）男博士生主要从事生物工程方面的研究． E-mail:liaojinsong＠yeah.net
作者简介:廖劲松（1976－）男博士生主要从事生物工程方面的研究．

Preparation and Fusion of the Strain Protoplast for the Degradation of Polyvinyl Alcohol

Liao Jin-song Guo Yong Zhuang Gui

1．College of Food and Biological EngineeringSouth China Univ．of Tech．Guangzhou510640GuangdongChina；
2．Dept．of Biological EngineeringZhengzhou Institute of Tech．Zhengzhou450052HenanChina

Received:2003-11-12 Online:2004-05-20 Published:2015-09-08
Contact: 廖劲松（1976－）男博士生主要从事生物工程方面的研究． E-mail:liaojinsong＠yeah.net
About author:廖劲松（1976－）男博士生主要从事生物工程方面的研究．

摘要/Abstract

摘要： 从自然界中分离得到4株能降解聚乙烯醇（PVA）的细菌经紫外线诱变得到两株具有单重抗药性的突变菌株S7（Str r Kan s ）和 K15（Str s Kan r ）．系统分析了各种因素对原生质体制备率以及再生率的影响；将S7和 K15作为亲本菌株进行原生质体融合并通过正交试验对原生质体融合的条件进行优化使融合率达到4．603×10－5 ．融合子F4菌株培养成活性污泥后PVA 降解率可达87％是普通活性污泥的3～4倍．

关键词: 原生质体融合, 菌种选育, 聚乙烯醇降解

Abstract: Four strains capable of degrading polyvinyl alcohol （PVA） were separated from natural specimensand two mutantsS7（Str r Kan s ） and K15（Str s Kan r ）which are of single drug resistancewere obtained via UV－irradia-tion．The effects of various factors on the preparation and the regeneration rates of the protoplast were systematically ana-lyzed．By the protoplast fusion of S7and K15as parent strains and via orthorhombic teststhe conditions of protoplast fu-sion were then optimizedin which the fusion rate was up to4．603×10－5 ．It is found that the fusion strain F4can clean 87％ of PVA when it is cultured into activated sludgewhich is3～4times that of ordinary activated sludge．

Key words: protoplast fusion, strain breeding, PVA degradation

中图分类号:

X172
X791

廖劲松郭勇庄桂 . 降解聚乙烯醇菌株原生质体的制备及融合[J]. 华南理工大学学报(自然科学版), 2004, 32(5): 74-79.

Liao Jin-song Guo Yong Zhuang Gui. Preparation and Fusion of the Strain Protoplast for the Degradation of Polyvinyl Alcohol[J]. Journal of South China University of Technology(Natural Science Edition), 2004, 32(5): 74-79.