华南理工大学学报(自然科学版) ›› 2004, Vol. 32 ›› Issue (5): 47-50,55.

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自养、异养和混养下小球藻的生长及生化成分

王海英 郭祀远 郑必胜 李存芝   

  1. 华南理工大学 轻化工研究所‚广东 广州510640
  • 收稿日期:2003-10-29 出版日期:2004-05-20 发布日期:2015-09-08
  • 通信作者: 王海英(1974-)‚女‚博士生‚主要从事微藻生物技术研究. E-mail:whyzxb@163.com
  • 作者简介:王海英(1974-)‚女‚博士生‚主要从事微藻生物技术研究.
  • 基金资助:
    广东省科技攻关项目(99M01409G)

Growth and Biochemical Components of Chlorella Vulgaris Under Autotrophic, Heterotrophic and Mixotrophic Cultivations

Wang Hai-ying  Guo Si-yuan  Zheng Bi-sheng  Li Cun-zhi   

  1. Research Institute of Light Industry and Chemical Engineering‚South China Univ.of Tech.,Guangzhou510640‚Guangdong‚China
  • Received:2003-10-29 Online:2004-05-20 Published:2015-09-08
  • Contact: Wang Ha- i ying (born in1974)‚female‚Ph.D.candidate‚mainly researches on microalgae biological technolo-gy. E-mail:whyzxb@163.com
  • About author:Wang Ha- i ying (born in1974)‚female‚Ph.D.candidate‚mainly researches on microalgae biological technolo-gy.
  • Supported by:
    Supported by the Key Research Grant from Science and Technology Committee of Guangdong Province(99M01409G)

摘要: 对小球藻在自养、异养和混养条件下的生长状况及细胞的生化成分(如可溶性蛋白、可溶性糖、叶绿素及脂肪酸组成)进行了研究.结果表明:异养和混养培养的小球藻的生物量远大于自养时的生物量;异养和混养培养的小球藻的比生长速率分别是自养时的2.13倍和3倍;光照对混养条件下的细胞生长有影响‚但光照强度为2.5klx 和4.0klx 时细胞生长的差别并不明显‚尤其在稳定生长期;与自养生长相比‚异养过程中小球藻的脂肪含量明显增加‚可溶性蛋白和可溶性糖的含量则有不同程度的降低‚叶绿素含量大大减少;异养有利于亚麻酸的积累;在混养条件下‚光照使可溶性蛋白、可溶性糖和叶绿素的含量增加‚其强度对细胞的生化成分有影响.

关键词: 小球藻, 生化成分, 生长, 自养, 异养, 混养

Abstract: Investigated in this paper are the growth and biochemical components‚such as soluble protein‚soluble sugar‚chlorophyll and fatty acid composition of Chlorella vulgaris‚under autotrophic‚heterotrophic and mixotrophic cultivations.The results show that the biomass of Chlorellavulgaris under heterotrophic and mixotrophic cultivations is much higher than that under autotrophic cultivation‚that the specific growth rates are respectively2.13and3times that under autotrophic growth‚and that the growth of cells under mixotrophic cultivation is affected by illuminence‚ but the effect is not significant at the illuminances of2.5and4.0klx‚especially in the stationary phase.Compared with autotrophic growth‚the content of fat under heterotrophic cultivation obviously increases‚while the contents of
soluble protein and saccharide decrease‚ especially chlorophyll. Heterotrophic cultivation is helpful to the accumulation of linolenic acid.The adoption of light makes soluble proteins‚saccharides and chlorophyll increase under mixotrophic growth‚and the illuminence also has effect on the biochemical component of Chlorella vulgaris.

Key words:  Chlorella vulgaris, biochemical component, growth, autotrophic cultivation, heterotrophic cultivation, mixotrophic cultivation

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