gD2 DNA 疫苗诱导细胞免疫应答

华南理工大学学报(自然科学版) ›› 2004, Vol. 32 ›› Issue (5): 42-46.

gD2 DNA 疫苗诱导细胞免疫应答

韩双艳¹ 郭勇¹ 杨慧兰² 王捷³ 杨太成³

1．华南理工大学食品与生物工程学院广东广州510640；2．广州军区广州总医院皮肤科广东广州510010；3．广州军区广州总医院医学实验科广东广州510010

收稿日期:2003-09-23 出版日期:2004-05-20 发布日期:2015-09-08
通信作者: 韩双艳（1976－）女博士生主要从事单纯疱疹病毒Ⅱ型 DNA 疫苗研究． E-mail:hanshuangyan＠21cn.com
作者简介:韩双艳（1976－）女博士生主要从事单纯疱疹病毒Ⅱ型 DNA 疫苗研究．
基金资助:
广东省自然科学基金资助项目（010795）

Cellular Immunity Induced by gD2 DNA Vaccine

Han Shuang-yan¹ Guo Yong¹ Yang Hui-lan² Wang Jie³Yang Tai-cheng³

1．College of Food and Biological EngineeringSouth China Univ．of Tech．Guangzhou510640GuangdongChina；
2．Dept．of DermatologyGuangzhou General Hospital of Guangzhou Military CommandGuangzhou510010GuangdongChina；
3．Dept．of Medical ResearchGuangzhou General Hospital of Guangzhou Military Command
Guangzhou510010GuangdongChina

Received:2003-09-23 Online:2004-05-20 Published:2015-09-08
Contact: Han Shuang-yan （born in1976）femalePh．D．candidatemainly researches on herpes simplex virus Ⅱ DNA vaccine． E-mail:hanshuangyan＠21cn.com
About author: Han Shuang-yan （born in1976）femalePh．D．candidatemainly researches on herpes simplex virus Ⅱ DNA vaccine．
Supported by:
Supported by Guangdong Provincial Natural Science Foundation （010795）

摘要/Abstract

摘要： 用生殖器疱疹 gD2DNA 疫苗 pcDNA3-gD 转染哺乳动物细胞 COS-7鉴定 gD 表达．再用其免疫BALB／c小鼠测定脾细胞增殖、CTL 细胞杀伤活性以及 CD4＋和 CD8 ＋细胞群．结果表明：pcDNA3-gD 能够在哺乳动物细胞COS-7中表达gD 抗原其免疫小鼠脾细胞增殖活性增加CTL 活性达37．69％明显高于 pcD-NA3组和生理盐水组；CD4＋占T 细胞数量的33．38％数量较 pcDNA3组和生理盐水组显著增加．

关键词: 免疫, gD2DNA疫苗, 细胞毒T 细胞, CD4＋, CD8＋, 疱疹

Abstract: pcDNA3-gDthe constructed Genital Herpes gD DNA vaccine （gD2DNA vaccine）was transfected into mammalian cell COS-7and the expression of gD gene was investigated．BALB／c mice were then immunized by pcDNA3-gD．The proliferation of spleen cellthe activity of cytotoxic T cell （CTL） and CD4＋ and CD8 ＋populations were respectively analyzed．The results show that pcDNA3-gD plasmid can express the gene of gD in cell COS-7that the spleen cell of mice immunized by pcDNA3-gD plasmid is of an increased proliferation activitythat the activity of CTL in the experiment group （immunized by pcDNA3-gD plasmid） is37．69％which is obviously higher than that of the control group （immunized by pcDNA3plasmid） and the naive group （immunized by normal saline）and that the percentage of CD4＋cells in the experiment group increases to33．38％ which is greater than that of the control group and the naive group．

Key words: immunity, gD2DNA vaccine, cytotoxic T cell, CD4＋, CD8＋, herpes

中图分类号:

Q789

韩双艳, 郭勇, 杨慧兰, 等. gD2 DNA 疫苗诱导细胞免疫应答[J]. 华南理工大学学报(自然科学版), 2004, 32(5): 42-46.

Han Shuang-yan, Guo Yong, Yang Hui-lan, et al. Cellular Immunity Induced by gD2 DNA Vaccine[J]. Journal of South China University of Technology(Natural Science Edition), 2004, 32(5): 42-46.