华南理工大学学报(自然科学版) ›› 2003, Vol. 31 ›› Issue (8): 82-87.

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利用扇贝边制造水解动物蛋白的研究

曾庆祝 曾庆孝 汪 涛 许庆陵   

  1. 1.华南理工大学 食品与生物工程学院‚广东 广州510640 2.大连水产学院 食品工程系‚辽宁 大连116023
  • 出版日期:2003-08-20 发布日期:2022-05-16

Manufacture of Hydrolyzed Animal Protein from Scallop Skirt 

Zeng Qing-zhu  Zeng Qing-xiao  Wang Tao  Xu Qing-ling    

  1. 1.College of Food and bioengineering South China Univ.of Tech. Guangzhou510640 China; 2.Department of Food Science and Engineering Dalian Fisheries University Dalian116023 China
  • Online:2003-08-20 Published:2022-05-16
  • About author:Zeng Qing-zhu(born in1965)‚male‚associate professor‚Ph.D.candidate‚mainly researches on seafood science.
  • Supported by:
    Supported by the Key Research Program of Guangdong province (2002C20314)

摘要: 选用复合蛋白酶酶解扇贝边制备水解液 并优化酶解工艺条件;采用中空纤维超滤膜对水解液进行分 离纯化 制造高质量的水解动物蛋白 并测试膜分离试验操作条件对分离效率的影响.研究结果表明 先用枯草 杆菌蛋白酶酶解4h 然后用风味蛋白酶继续酶解 可以显著提高酶解液中多肽、短肽及游离氨基酸的含量;超 过滤的操作压力、温度、酶解液的 pH 值及浓缩比等因素都将影响超过滤效率 在操作压力不高于0.10MPa pH 值为7.0~7.5 温度为43℃ 每过滤10min 后就对超过滤膜进行2min 的反冲洗等操作条件下 可以大大提高 超过滤的分离效率、减少膜的污染及延长膜的使用寿命;试验结果还表明 超过滤分离后水解液的感官指标明 显优于未分离的水解原液. 

关键词: 水解动物蛋白, 扇贝边, 酶解, 中空纤维超滤

Abstract:  After hydrolysis with mixed protease the hydrolysates of scallop skirt was purified by hollow fiber ultrafiltration(UF).The processing parameters for the manufacture of liquid-HAP in good quality were investigated.Results show that the content of polypeptides peptides and free amino acids in the liquid-HAP is largely increased by using flavorzyme after4h pre-hydrolysis with A.S.1.398neutral subtilisin.And that permeation flux was affected by operating pressure temperature pH value of hydrolysates and the ratio of volume to concentration (RVC). Optimal operative condition which might prolong working time of the device with high permeation flux minimize pollution of membranes and raise separating efficiency is pH7.0~7.5at 43℃ under0.10MPa and the membranes rinsed repeatedly for2minutes after every operating for10minutes.These results suggest that sense index of fractionated hydrolysates is superior to that of the original hydrolysates. 

Key words: hydrolyzed animal protein, scallop skirt, enzymatic hydrolysis, hollow fiber ultrafiltration

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