关键词: 冷藏肉品, 三重荧光ERA, 食源性致病菌, 快速检测

Abstract:

Meat is rich in nutrients and easy to breed microorganisms. Even at low temperatures, many microorganisms remain active and retain some virulence, so it is still necessary to screen for pathogens. In this study, a triple fluorescence ERA method was developed for the first time to detect Staphylococcus aureus, Salmonella and Listeria monocytogenes in meat products based on enzymatic recombinase amplification (ERA) technique. After optimization, the method could complete the simultaneous detection of three pathogens within 12 min. From DNA extraction to ERA detection, the whole detection process only takes 30 min, with the minimum detection limit of 10^-2 ng/μL. The results of artificial pollution simulation showed that the method could detect three pathogens at the same time after the meat samples with 1 CFU/mL of pollution were cultured for 6 hours. The results of commercially available refrigerated meat products showed that the detection rate of three pathogens was between 6.45% and 19.35%, which was consistent with the detection results of the real-time PCR method of SN/T1870—2016. It was confirmed that the method established in this study has high accuracy and feasibility. This study will help reduce the potential disease caused by foodborne pathogens, and lays a certain foundation for on-site rapid detection of foodborne pathogens in the future.

Key words: refrigerated meat products, triple fluorescence ERA, foodborne pathogens, rapid detection