In order to implement the high-valued utilization of tobacco stem,supercritical CO2 fluid extraction was conducted to extract the flavor components from waste tobacco stem and to prepare essential oil with natural tobacco flavors.Then,by taking the extraction yield as an evaluation index,single-factor and orthogonal experiments were respectively carried out to optimize the extraction process,Finally,the flavor components in the obtained extracts were analyzed by means of GC-MS.The results show that,under the optimal extraction conditions,that is,an ex- traction temperature of 60 ℃,an extraction pressure of 32 MPa,a CO2 flow rate of 24 L/h,an extraction time of 2. 5 h,when 85% ethanol solution with a mass fraction in tobacco stem of 20% is adopted as the entrainer,the ex- traction yield reaches 1. 70%; and that many important flavor components are contained in the 27 kinds of identi- fied components,such as β-damascenone,megastigmatrienone,β-cembrenediol,geranyl acetone,neophytadiene,and so on.