The extraction of Eucalyptus urophydis (E. urophydis) wood consumes a lot of bleaching agents and other additives, aggravates the environmental pollution due to wastewater, and causes a great consumption of extractives. In order to solve these problems, Py-GC/MS was used to analyze the main chemical components of benzene/ ethanol extractives from flesh and old E. urophydis chips. The results show that there are 145 compounds in the benzene/ethanol extractives from flesh E. urophydis chip, including 1-phenanthrenecarboxylic acid, 1,2,3,4,4α, 9,10, 10α-octahydro-1,4α-dimethyl-7-( 1-methylethyl )-, methyl ester ( 19.49% ), 3,7-dihydroxy-9-methoxy-1- methyl-6H-dibenzo [ B, D ] pyran- 6-one ( 5.01% ), 1-phenanthrenecarboxylic acid, 1,2,3,4,4α, 9,10,10a-octa- hydro-1,4α-dimethyl-7-( 1-methylethyl) (4. 47% ), ergosta-4,6,22-trien-3β-ol (4. 07% ), phenol ,2,6-dimethoxy (3. 94% ) , hexadecanoic acid (2. 96% ), acetic acid (2.86%), ethanone ,1-(3-methoxyphenyl) (2. 83% ), phenol ,2- methoxy (2.47%), germanicol (2. 00% ), etc. , and that there are 55 compounds in the extractives from old E. urophydis chip, including ergosta-4,6,22-trien-3β-ol (23.33%), brucine (9.61%), chol-8 (14)-en-24-ol, (5β) (6.86%), oleic acid (6. 14% ), phenol, 2-methyl (5.63%), 1-mercapto-2-heptadecanone (4. 06% ), 2,4- dibenzyl-5,8-dimethoxy-6-methyl-l-naphthol (3.86%), hexadecanoic acid (3.65%),β-sitosterol (3.12%), 4- chloro-2-fluoroaniline (2. 29% ), 9,19-cycloergost-24 (28) -en-3-ol,4,14-dimethyl-, (3β,4α ,5α) (2. 19% ), 7- heptadecene, 17-chloro (2.09%), etc. It is indicated that the benzene/ethanol extractives from both fresh and old E. uropydis chips are of potential application prospects in the fields of biological energy, biological medicine, cosmetic, skin care and flavor.