Biological Engineering

Transformation of Polygonum cuspidatum Resveratrol Synthase Gene into Arabidopsis thaliana

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  • 1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;2.College of Life Science,South China Agricultural University,Guangzhou 510642,Guangdong,China;3.General Hospital of Guangzhou Military Command,Guangzhou 510010,Guangdong,China
柳忠玉(1978-),女,博士生,主要从事药用植物次生代谢研究

Received date: 2010-10-12

  Revised date: 2010-12-29

  Online published: 2011-04-01

Supported by

广东省自然科学基金资助项目(10151001002000012);广东省科技计划项目(2010B060200009

Abstract

In order to reveal the effectiveness of Polygonum cuspidatum resveratrol synthase(PcRS) gene in improving the resveratrol accumulation in foreign species,first,the plant expression vector pCAM1380-35S-PcRS was constructed and introduced into Arabidopsis thaliana by Agrobacterium tumefaciens by means of the floral dip me-thod.Next,the transformants were screened on the medium containing hygromycin.Then,the integration of PcRS gene into the transgenic plants was verified via the PCR and the Southern blotting.Moreover,independent homozygous transgenic Arabidopsis thaliana lines with genetical stability were obtained after the selection of T3 progenies,and the expression of the gene transferred into Arabidopsis thaliana was confirmed by Northern blotting.Finally,an end product,namely trans-piceid,was identified by HPLC,the content of which in 2-week-old plants being 136 μg/g(fresh weight) or 1 813 μg/g(dry weight).

Cite this article

Liu Zhong-yu Zhuang Chu-xiong Sheng Shu-jing Shao Li Zhao Shu-jin . Transformation of Polygonum cuspidatum Resveratrol Synthase Gene into Arabidopsis thaliana[J]. Journal of South China University of Technology(Natural Science), 2011 , 39(5) : 138 -142 . DOI: 10.3969/j.issn.1000-565X.2011.05.024

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