Journal of South China University of Technology(Natural Science) >

2010 , Vol. 38 >Issue 12: 145 - 150

DOI: https://doi.org/10.3969/j.issn.1000-565X.2010.12.028

Biological Engineering

Purification and Structure Identification of Phytohemagglutinin-L4

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  • 1.Industrial Technology Research Institute,South China University of Technology,Guangzhou 510640,Guangdong,China;2.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;3.School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China;4.Guangzhou Hezhu Bio-Tech Co.,Ltd.,Guangzhou 510633,Guangdong,China
张晓元（1964-），男，博士，副教授，主要从事生物医药、天然产物研究．

Received date: 2010-01-04

  Revised date: 2010-06-01

  Online published: 2010-12-25

Supported by

中国博士后基金资助项目（2004036497）; 广东省科技计划项目（2005B33001008）

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Abstract

Mitogenetic glycoprotein PHA-L4 with high activity was obtained from phytohemagglutinin（PHA） after the separation and purification by means of anion exchange chromatography and biological activity inspection.The structure of the product was then identified by means of SDS-PAGE,mass spectrometry,N-terminal sequencing measurement and peptide mass fingerprint.The results indicate that PHA-L4 is a kind of glycoprotein tetramer composed of 4 PHA-L subunits,with a relative molecular mass of 120000,and that each subunit is heterogeneously glycosylated at the 12th Asn to a certain degree.

Key words： phytohemagglutinin; purification; identification; mass spectrometry; N-terminal sequencing; peptide mass fingerprint

Cite this article

Zhang Xiao-yuan Guo Yong Wu Hui Zhao Qi Huang Min . Purification and Structure Identification of Phytohemagglutinin-L4[J]. Journal of South China University of Technology(Natural Science), 2010 , 38(12) : 145 -150 . DOI: 10.3969/j.issn.1000-565X.2010.12.028

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