In order to obtain the suspension cell line of Polygonum multiflorum Thunb.and investigate the suspension culture and stilbene glycoside metabolic pathways,callus must be obtained.In this paper,the root and the stem of Polygonum multiflorum Thunb.were used as explants to acquire the most suitable callus induction medium via orthogonal tests.It is found that the root and the stem are most appropriately induced on the MS medium containing 1mg/L 2,4-dichlorophenoxyacetic acid and 0.4mg/L naphthaleneacetic acid at 26℃ in dark,with the induction rate being up to 96.7%.Moreover,HPLC results indicate that the contents of stilbene glycoside in root and stem callus are respectively 1.5 and 0.4mg/g.