Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (9): 147-150.doi: 10.3969/j.issn.1000-565X.2010.09.028

• Biological Engineering • Previous Articles    

Contents of Stilbene Glycoside in Root and Stem Callus of Polygonum multiflorum Thunb

Shao Li 1  Cui Tang-bing1  Zhao Shu-jin2   

  1. 1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;2 General Hospital of Guangzhou Military Command,Guangzhou 510010,Guangdong,China
  • Received:2010-01-19 Revised:2010-03-26 Online:2010-09-25 Published:2010-09-25
  • Contact: 赵树进（1958-），男，教授，博士生导师，主要从事药学研究．E-mail：gzzsjzhs@163．com. E-mail:qssl@163．com
  • About author:邵利（1978-），男，博士生，主要从事生物制药研究．
  • Supported by:

    广东省自然科学基金资助项目（06019716）

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Abstract:

In order to obtain the suspension cell line of Polygonum multiflorum Thunb.and investigate the suspension culture and stilbene glycoside metabolic pathways,callus must be obtained.In this paper,the root and the stem of Polygonum multiflorum Thunb.were used as explants to acquire the most suitable callus induction medium via orthogonal tests.It is found that the root and the stem are most appropriately induced on the MS medium containing 1mg/L 2,4-dichlorophenoxyacetic acid and 0.4mg/L naphthaleneacetic acid at 26℃ in dark,with the induction rate being up to 96.7%.Moreover,HPLC results indicate that the contents of stilbene glycoside in root and stem callus are respectively 1.5 and 0.4mg/g.

Key words: Polygonum multiflorum Thunb, callus, high-pressure liquid chromatography, stilbene glycoside