Journal of South China University of Technology(Natural Science) >

2024 , Vol. 52 >Issue 7: 81 - 87

DOI: https://doi.org/10.12141/j.issn.1000-565X.230661

Chemistry & Chemical Engineering

Process Research on Supercritical CO2 Fluid Extraction of Natural Nicotine from Tobacco

  • ZHANG Huiping ,
  • TANG Qianru ,
  • YAN Ying
Expand
  • School of Chemistry and Chemical Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China
张会平（1964—），男，教授，博士生导师，主要从事精细化工等研究。E-mail： hpzhang@scut.edu.cn

Received date: 2023-10-25

  Online published: 2023-12-28

Supported by

the National Natural Science Foundation of China(22178122)

Fold

Abstract

Extracting nicotine from tobacco can achieve the resource utilization of waste tobacco. Therefore, this article conducted a single-factor experiment to investigate the effects of process conditions (extraction time, extraction pressure, extraction temperature, CO2 flow rate, particle size of raw materials) on the yields of nicotine and tobacco extract. In addition, using nicotine extraction rate as the indicator, extraction time, extraction pressure, extraction temperature, and CO2 flow rate as factors, an orthogonal experiment was designed to optimize the extraction process. The composition of the obtained tobacco extract was analyzed with gas chromatography-mass spectrometry. The results show that extraction temperature has the greatest impact on nicotine extraction rate, extraction time and pressure have a relatively large impact, and CO2 flow rate has the least impact. The optimal extraction conditions are as follows: extraction temperature is 65 ℃, extraction pressure is 22 MPa, CO2 flow rate is 25 L/h (based on 150.0 g of tobacco raw material), extraction time is 80 minutes, and particle size is 0.60 mm. Under the optimal conditions, the nicotine extraction rate was 97.89%, and the tobacco extract yield was 5.57%. Fourteen components were detected in the volatiles of the tobacco extract, and the main components were nicotine (52.22%), neophytadiene (10.29%), and vitamin E (6.67%). Additionally, the extract also contained phytosterols, linoleic acid, and liquidambar resin, etc. Due to high nicotine content, the extract can be used as a good raw material for nicotine purification in later stages.

Key words： nicotine; supercritical fluid; extraction; orthogonal experiment

Cite this article

ZHANG Huiping , TANG Qianru , YAN Ying . Process Research on Supercritical CO2 Fluid Extraction of Natural Nicotine from Tobacco[J]. Journal of South China University of Technology(Natural Science), 2024 , 52(7) : 81 -87 . DOI: 10.12141/j.issn.1000-565X.230661

References

1 任民，王志德，牟建民，等 ．我国烟草种质资源的种类与分布概况［J］．中国烟草科学，2009，30（S1）：8-14.
  REN Min， WANG Zhide， MU Jianmin，et al ．Overview of species and distribution of tobacco germplasm resources in China［J］．Chinese Tobacco Science，2009，30（S1）：8-14.
2 张骞方，叶鹏盛，代顺冬，等 ．烟草生产废弃物资源化利用现状及建议［J］．现代农业科技，2020（3）：181，183.
  ZHANG Qianfang， YE Pengsheng， DAI Shundong，et al ．Current situation and suggestions on the resource utilization of tobacco waste［J］．Modern Agricultural Science and Technology，2020（3）：181，183.
3 徐晓铭，张洋，司成，等 ．烟草提取物的药理作用研究进展［J］．山东医药，2023，63（11）：112-115.
  XU Xiaoming， ZHANG Yang， SI Cheng，et al ．Research progress on the pharmacological effects of tobacco extracts［J］．Shandong Medical Journal，2023，63（11）：112-115.
4 高宏建，张献忠，钟建军，等 ．水蒸汽蒸馏法提取烟草精油的研究［J］．食品工业科技，2011，32（10）：388-390.
  GAO Hong-jian， ZHANG Xian-zhong， ZHONG Jian-jun，et al ．Research on extraction tobacco essential oils by steam distillation［J］．Science and Technology of Food Industry，2011，32（10）：388-390.
5 ZHANG X， GAO H， ZHANG L，et al ．Extraction of essential oil from discarded tobacco leaves by solvent extraction and steam distillation，and identification of its chemical composition［J］．Industrial Crops and Products，2012，39：162-169.
6 刘春波，于昊辰，李易丞，等 ．从烟叶中提取烟碱的工艺条件优化［J］．辽宁化工，2019，48（1）：24-26.
  LIU Chun-bo， YU Hao-chen， LI Yi-cheng，et al ．Optimization of extraction process of nicotine from tobacco leaves［J］．Liaoning Chemical Industry，2019，48（1）：24-26.
7 BANO?I? M， BANJARI I， FLANJAK I，et al ．Optimization of MAE for the separation of nicotine and phenolics from tobacco waste by using the response surface methodology approach［J］．Molecules，2021，26（14）：1-14.
8 王莹，杨海朝，刘凤霞，等 ．微波和超声波辅助萃取废弃烟叶及烟杆中烟碱的研究［J］．农产品加工，2019（10）：1-5.
  WANG Ying， YANG Haichao， LIU Fengxia，et al ．Study on extraction of nicotine from discarded tobacco leaves and stems by microwave and ultrasonic assisted extraction［J］．Farm Products Processing，2019（10）：1-5.
9 M?CK D M J， RIEGERT C， RADTKE S，et al ．Process optimization and extraction of acids，syringols，guaiacols，phenols and ketones from beech wood slow pyrolysis liquids with supercritical carbon dioxide at different densities［J］．The Journal of Supercritical Fluids，2023，199：105937/1-13.
10 PEREIRA G S L， MAGALH?ES R D S， FRAGA S，et al ．Extraction of bioactive compounds from Butia capitata fruits using supercritical carbon dioxide and pressurized fluids［J］．The Journal of Supercritical Fluids，2023，199：105959/1-7.
11 PINTO M B C， VARDANEGA R， NáTHIA-NEVES G，et al ．Novel Brazilian hop （Humulus lupulus L.） extracts through supercritical CO2 extraction：enhancing hop processing for greater sustainability［J］．Food Research International，2023，172：1-12.
12 PAN Z， WANG X， LI Q，et al ．Supercritical extraction technique of agarwood essential oil induced by plant hormones［J］．BioResources，2023，18（4）：6741-6760.
13 MATEUS L S， DUTRA J M， FAVARETO R，et al ．Optimization studies and compositional oil analysis of pequi （caryocar brasiliense cambess） almonds by supercritical CO2 extraction［J］．Molecules，2023，28（3）：1-13.
14 赵丹，尹洁 ．超临界流体萃取技术及其应用简介 ［J］．安徽农业科学，2014，42（15）：4772-4780.
  ZHAO Dan， YIN Jie ．Overview of supercritical fluid extraction and its application［J］．Journal of Anhui Agricultural Sciences，2014，42（15）：4772-4780.
15 吴芳，李雄山，陈乐斌 ．超临界流体萃取技术及其应用［J］．广州化工，2018，46（2）：21-23.
  WU Fang， LI Xiong-shan， CHEN Le-bin ．Application of supercritical fluid extraction［J］．Guangzhou Chemical Industry，2018，46（2）：21-23.
16 邱运仁，俞晓惠，杜吉华 ．超临界CO2萃取烟叶中的烟碱［J］．烟草科技，2006（8）：21-24.
  QIU Yun-ren， YU Xiao-hui， DU Ji-hua ．Extraction of nicotine in tobacco with supercritical carbon dioxide fluid［J］．Tobacco Science & Technology，2006（8）：21-24.
17 杨靖，陈芝飞，孙志涛 ．超临界CO2流体萃取烟叶中烟碱工艺研究［J］．香料香精化妆品，2010（1）：17-18.
  YANG Jing， CHEN Zhifei， SUN Zhitao ．Extraction technology on nicotine from tobacco by supercritical CO2 ［J］．Flavour Fragrance Cosmetics，2010（1）：17-18.
18 DJAPIC N ．Supercritical carbon dioxide extraction of nicotiana tabacum leaves：optimization of extraction yield and nicotine content［J］．Molecules，2022，27（23）：1-11.
19 DONG Z B， SHAO W Y， LIANG Y R ．Isolation and characterization of essential oil extracted from tangerine peel ［J］．Asian Journal of Chemistry，2014，26（16）：4975-4978.
20 阳元娥，谭伟，李桂锋 ．超临界CO2萃取废次烟叶中烟碱工艺优化［J］．化学与生物工程，2008，25（6）：74-76.
  YANG Yuan-e， TAN Wei， LI Gui-feng ．Optimization of extraction technology of nicotine from waste tobacco leaves by supercritical CO2 ［J］．Chemistry and Bioengineering，2008，25（6）：74-76.
21 RAJPUT S， KAUR S， PANESAR P S，et al ．Supercritical fluid extraction of essential oils from Citrus reticulata peels：optimization and characterization studies［J］．Biomass Conversion and Biorefinery，2023，13（16）：14605-14614.
Options
Outlines

/

〈 〉