Journal of South China University of Technology(Natural Science) >

2024 , Vol. 52 >Issue 4: 33 - 41

DOI: https://doi.org/10.12141/j.issn.1000-565X.230082

Food Science & Technology

Inhibitory Effects and Molecular Mechanism of Wheat Antioxidant Peptides on Oxidative Stress Injury in Human Embryonic Kidney Cells

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  • 1.College of Engineering，China Agricultural University，Beijing 100083，China
    2.Beijing Engineering Research Center of Protein and Functional Peptides，China National Research Institute of Food and Fermentation Industries Co. ，Ltd. ，Beijing 100015，China
刘文颖（1984-），女，博士生，高级工程师，主要从事农产品加工工程研究。E-mail:wenyingliu888@126.com

Received date: 2023-03-03

  Online published: 2023-08-16

Supported by

the Key R&D Program of Ningxia Hui Autonomous Region(2021BEG02027)

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Abstract

The study firstly established oxidative stress injury model of human embryonic kidney cells (HEK293) by the induction of 2,2-azobis (2-methylpropionamidine) dihydrochloride (AAPH) to evaluate the antioxidant activity of five wheat protein-derived peptides Leu-Tyr (LY), Pro-Tyr (PY), Tyr-Gln (YQ), Ala-Pro-Ser-Tyr (APSY) and Arg-Gly-Gly-Tyr (RGGY). Then, it used quantum chemistry and molecular docking techniques to predict the optimal configuration and binding effect of five wheat protein-derived peptides combined with 2,2-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), and to explore the molecular mechanism of wheat protein-derived peptides. The results of cell test show that after the action of five wheat protein-derived peptides, the cell death rate significantly decreases to below 3.68% (P < 0.05), and the generation of reactive oxygen species (ROS) induced by AAPH was significantly reduced (P < 0.05), making the ROS content tend to normal levels. All five wheat protein-derived peptides show good total antioxidant capacity and free radical scavenging capacity of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) (P < 0.05). RGGY shows the strongest total antioxidant capacity, with an activity value of (1.46 ± 0.08) mmol/L Trolox, followed by APSY, YQ, PY and LY. The DPPH free radical scavenging ability of YQ is the strongest, with a scavenging rate of 61.34% ± 2.24%, followed by APSY, RGGY, PY and LY. The results of molecular docking show that the CDOCKER interaction energy (-CIE) scores of the five wheat protein-derived peptides are 13.304 9, 13.397 3, 13.412 1, 16.768 5 and 16.268 3, respectively, which can effectively interact with ABTS, mainly through the formation of strong hydrogen bonds and hydrophobic forces between ABTS molecules to exert antioxidant activity.

Key words： wheat protein-derived peptide; antioxidant; cell culture; molecular docking technology; molecular mechanism

Cite this article

LIU Wenying, REN Jie, WU Hanshuo, et al . Inhibitory Effects and Molecular Mechanism of Wheat Antioxidant Peptides on Oxidative Stress Injury in Human Embryonic Kidney Cells[J]. Journal of South China University of Technology(Natural Science), 2024 , 52(4) : 33 -41 . DOI: 10.12141/j.issn.1000-565X.230082

References

1 ZHAO X C， ZHANG L， YU H X，et al ．Curcumin protects mouse neuroblastoma Neuro-2A cells against hydrogen-peroxide-induced oxidative stress［J］．Food Chemistry，2011，129（2）：387-394.
2 LUSHCHAK V I ．Free radicals，reactive oxygen species，oxidative stress and its classification［J］．Chemico-Biological Interactions，2014，224：164-175.
3 YI G F， DIN J U， ZHAO F，et al ．Effect of soybean peptides against hydrogen peroxide induced oxidative stress in HepG2 cells via Nrf2 signaling［J］．Food & Function，2020，11（3）：2725-2737.
4 刘文颖，冯晓文，程青丽，等 ．大米低聚肽的制备和结构表征及体外抗氧化作用［J］．华南理工大学学报（自然科学版），2021，49（11）：47-56.
  LIU Wenying， FENG Xiaowen， CHENG Qingli，et al ．Preparation，structure characterization and in vitro antioxidant activity of rice oligopeptides［J］．Journal of South China University of Technology （Natural Science Edition），2021，49（11）：47-56.
5 NORDBERG J， ARNER E S J ．Reactive oxygen species，antioxidants，and the mammalian thioredoxin system［J］．Free Radical Biology and Medicine，2001，31（11）：1287-1312.
6 吉正梅，张晓春，彭钰迪，等 ．鸭胚源抗氧化肽TD12对HepG2细胞氧化应激损伤的保护作用［J］．食品与发酵工业，2021，47（18）：141-148.
  JI Zhengmei， ZHANG Xiaochun， PENG Yudi，et al ．Protective effect of duck embryo-derived antioxidant peptide TD12 on oxidative stress damage in HepG2 cells ［J］．Food and Fermentation Industries，2021，47（18）：141-148.
7 KARAMI Z， PEIGHAMBARDOUST S H， HESARI J，et al ．Antioxidant，anticancer and ACE-inhibitory activities of bioactive peptides from wheat germ protein hydrolysates［J］．Food Bioscience，2019，32：100450/1-12.
8 ZHANG J， WEN C， LI C，et al ．Antioxidant peptide fractions isolated from wheat germ protein with subcritical water extraction and its transport across Caco-2 cells［J］．Journal of Food Science，2019，84（8）：2139-2146.
9 LIU W Y， ZHANG J T， TAKUYA MIYAKAWA，et al ．Antioxidant properties and inhibition of angiotensin-converting enzyme by highly active peptides from wheat gluten［J］．Scientific Reports，2021，11（1）：5206/1-11.
10 张燕，陈志飞，赵颂宁，等 ．蛋清源抗氧化肽对HEK293细胞氧化应激损伤的抑制作用及机制［J］．中国食品学报，2019，19（10）：11-22.
  ZHANG Yan， CHEN Zhifei， ZHAO Songning，et al ．The anti-oxidative effects and mechanism of antioxidant peptides from egg white against oxidative stress injury in human embryonic kidney 293 cells［J］．Journal of Chinese Institute of Food Science and Technology，2019，19（10）：11-22.
11 ZHANG Q， CUI C， CHEN C Q，et al ．Anti-proliferative and proapoptotic activities of Alpinia oxyphylla on HepG2 cells through ROS-mediated signaling pathway［J］．Journal of Ethnopharmacology，2015，169：99-108.
12 LIU E Y， FANG L， FENG X W，et al ． In vitro antioxidant and angiotensin I-converting enzyme inhibitory properties of peptides derived from corn gluten meal ［J］．European Food Research and Technology，2020，246：2017-2027.
13 MINE Y， MA F P， LAURIAU S ．Antimicrobial peptides released by enzymatic hydrolysis of hen egg white lysozyme［J］．Journal of Agricultural and Food Chemistry，2004，52（5）：1088-1094.
14 WEN C， ZHANG J， ZHANG H，et al ．Study on the structure-activity relationship of watermelon seed antioxidant peptides by using molecular simulations［J］．Food Chemistry，2021，364：130432/1-7.
15 ZHANG J， LI M， ZHANG G，et al ．Identification of novel antioxidant peptides from snakehead （Channa argus） soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms［J］．Food Chemistry，2021，337：127921/1-11.
16 LI C， ZHAN Y D， MA X Z，et al ．B7-H4 facilitates proliferation and metastasis of colorectal carcinoma cell through PI3K/Akt/mTOR signaling pathway［J］．Clinical and Experimental Medicine，2020，20（1）：79-86.
17 LIU L， QIU T X， SONG D W，et al ．Inhibition of a novel coumarin on an aquatic rhabdovirus by targeting the early stage of viral infection demonstrates potential application in aquaculture［J］．Antiviral Research，2020，174：104672/1-13.
18 LU Q B， LIN X Y， WU J，et al ．Matrine attenuates cardiomyocyte ischemia-reperfusion injury through activating AMPK/Sirt3 signaling pathway［J］．Journal of Receptors and Signal Transduction Research，2021，41（1）：488-493.
19 LU J M， LIN P H， YAO Q Z，et al ．Chemical and molecular mechanisms of antioxidants：experimental approaches and model systems［J］．Journal of Cellular and Molecular Medicine，2010，14（4）：840-860.
20 NEMOTO S， TAKEDA K， YU Z X，et al ．Role for mitochondrial oxidants as regulators of cellular metabolism［J］．Molecular and Cellular Biology，2000，20（19）：7311-7318.
21 YU D L， ZHA Y Y， ZHONG Z，et al ．Improved detection of reactive oxygen species by DCFH-DA：new insight into self-amplification of fluorescence signal by light irradiation［J］．Sensors and Actuators B：Chemical，2021，339：129878/1-9.
22 LIU X， FU Q， XU P，et al ．Rapid determination of monopersulfate with bromide ion-catalyzed oxidation of 2，2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid （ABTS）［J］．Chemical Engineering Journal，2022，433：133551/1-8.
23 QIN X Y， ZHANG J T， LIU G M，et al ．Structure and composition of a potential antioxidant obtained from the chelation of pea oligopeptide and sodium selenite［J］．Journal of Functional Foods，2019，64：103619/1-8.
24 CHANG O K， HA G E， HAN G S，et al ．Novel antioxidant peptide derived from the ultrafiltrate of ovomucin hydrolysate［J］．Journal of Agricultural and Food Chemistry，2013，61：7294-7300.
25 SUN J， HE H， XIE B J ．Novel antioxidant peptides from fermented mushroom Ganoderma lucidum［J］．Journal of Agricultural and Food Chemistry，2004，52：6646-6652.
26 CHEN H M， MURAMOTO K， YAMAUCHI F，et al ．Structural analysis of antioxidative peptides from soybean beta-conglycinin［J］．Journal of Agricultural and Food Chemistry，1995，43：574-578.
27 SAITO K， JIN D H， OGAWA T，et al ．Antioxidative properties of tripeptide libraries prepared by the combinatorial chemistry［J］．Journal of Agricultural and Food Chemistry，2003，51：3668-3674.
28 GU R Z， LIU W Y， LIN F，et al ．Antioxidant and angiotensin I-converting enzyme inhibitory properties of oligopeptides derived from black-bone silky fowl （Gallus gallus domesticus Brisson） muscle［J］．Food Research International，2012，49：326-333.
29 ZHOU C， HU J， MA H，et al ．Antioxidant peptides from corn gluten meal：orthogonal design evaluation ［J］．Food Chemistry，2015，187（15）：270-278.
30 ZHU K X， ZHOU H M， QIAN H F ．Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates （WGPH） prepared with alcalase［J］．Process Biochemistry，2006，41（6）：1296-1302.
31 张燕，魏汝君，潘风光，等 ．蛋清源活性肽抗氧化及抗炎活性［J］．食品科学，2018，39（13）：153-158.
  ZHANG Yan， WEI Rujun， PAN Fengguang，et al ．Antioxidant and anti-inflammatory effects of bioactive peptides derived from egg white proteins［J］．Food Science，2018，39（13）：153-158.
32 WU R， HUANG J， HUAN R，et al ．New insights into the structure-activity relationships of antioxidative peptide PMRGGGGYHY［J］．Food Chemistry，2021，337：127678/1-8.
33 文超婷 ．西瓜籽肽的抗氧化构效关系及其分子机制研究［D］．镇江：江苏大学，2021.
34 YANG Q， CAI X， YAN A，et al ．A specific antioxidant peptide：its properties in controlling oxidation and possible action mechanism［J］．Food Chemistry，2020，327：126984/1-9.
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