The inhibition of fibrinogen ( FIB) to fibrin， the activated partial thromboplastin time ( APTT) ， the prothrombin time ( PT) and the thrombin time ( TT) were used to study the anticoagulant activity of acid-Auricularia auricular polysaccharides which were obtained by three kinds of physical-assisted extraction techniques，ultrasound，microwave and high shear． And then the correlation between the extraction technique and the anticoagulant activity was analyzed by testing the structures and physicochemical properties of polysaccharides． The results showed that the acid-Auricularia auricula polysaccharides had a significant inhibitory effect on the transformation of FIB，and it could also prolong the APTT and TT of rabbit plasma ( p ＜0. 05) significantly，but it produced no significant effect on PT ( p ＞0. 05) ，so it is through endogenous pathway and common pathway to involve itself in the coagulation process． The polysaccharides with the best anticoagulant activity in each treatment were selected for further comparative analysis，and the W-Ac-AAP，which was obtained by microwave-assisted extraction for 15 min ( W-Ac-AAP) ，had the best anticoagulant activity． When the concentration of polysaccharide at 4. 0 g/L，the APTT was ( 125. 03 ±1. 64) s and the TT was ( 70. 39 ±0. 87) s． When the concentration of polysaccharide at 2. 0 g/L， the ability of FIB to inhibit fibrin conversion was ( 0. 76 ±0. 05) g/L． There had been significant differences in protein content and sulfate content among the four polysaccharides ( p ＜ 0. 05) ． The W-Ac-AAP with the best anticoagulant activity had the lowest protein content， the highest sulfate content，and polysaccharides with common alkali extraction ( Ac-AAP) had the lowest sulfate content， there would be a certain correlation between the anticoagulant activity and sulfate content of polysaccharides． This study provides a means for the development of natural plant anticoagulant drugs．