Lactobacillus sp.DMDL 9010 was isolated from fermented vegetables and was used for the degradation ofnitrites in MRS broth,and the results are compared with those of other four lactic acid bacteria including LCR 719,LB 1.83,ST 1.204 and LP 8140,with the best nitrite degradation effect of DMDL 9010 after 24- h fermentation(P≤0.001) being revealed. Then,the identification scope of DMDL 9010 was reduced to Lactobacillus plantarumand Lactobacillus pentosus with the help of 16S rDNA sequence,and the strain was identified as L.pentosus by tes-ting physiological and biochemical characters,although no specific stripe was obtained in the subsequent PCR.More-over,a homologous DNA sequence of L- lactate dehydrogenase 1 (ldhL1) with a similarity of 90% was found in thegenome of L.pentosus and L.plantarum,and the low DNA sequence similarity in the upstream and downstreamfragments of ldhL1 could be regarded as an identification criterion of the two strains.Finally,after the PCR amplifi-cation and sequencing of the upstream and downstream fragments of ldhL1,the above- mentioned sequences werecompared via the bioinformatics analysis,and DMDL 9010 was identified as L.plantarum.