Journal of South China University of Technology(Natural Science) >

2015 , Vol. 43 >Issue 11: 8 - 15

DOI: https://doi.org/10.3969/j.issn.1000-565X.2015.11.002

Food Science & Technology

Separation，Identification and Antioxidant Activity of Glycoproteins from Millettia Speciosa Champ．

  • ZHAO Qiang-Zhong ,
  • FENG Meng-Ying ,
  • LIN Lian-Zhu ,
  • ZHAO Mou-Ming
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  • School of Light Industry and Food Sciences，South China University of Technology，Guangzhou 510640，Guangdong，China
赵强忠(1976-)，男，博士，教授，博士生导师，主要从事食品生物技术研究． E-mail: qzzhao@ scut． edu． cn

Received date: 2015-04-10

  Revised date: 2015-06-24

  Online published: 2015-10-01

Supported by

Supported by the Production，Education and Ｒesearch Cooperative Project of Guangdong Province and the Ministry
of Education(2012B090600025)

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Abstract

In order to understand well the structural characteristics，physical and chemical properties and biologi-cal activities of glycoprotein from Millettia Speciosa Champ． (MSCG)，MSCG was purified by means of ultrafiltra-tion，chromatography Sephadex G-75 and ODS according to its molecular mass distribution，and thus two purified fractions MSCG-1 and MSCG-2 were obtained． Then，the structures of the two fractions were identified by means of HPLC，GC，GC-MS and FT-IＲ． The results show that (1) the molecular masses of MSCG-1 and MSCG-2 are 62. 00 ku and 1. 94 ku respectively; (2) MSCG-1 contains 50. 22% protein and 38. 07% sugar，and it is mainly composed of → 4，6)-Galp-(1 → and Araf-(1 → ; (3) MSCG-2 contains 69. 37% protein and 6. 70% sugar，and its main chain is composed of → 6)-Glcp-(1 → and has a high branch degree; (4) MSCG-1 and MSCG-2 both con-tain O-link bonds; (5) for the amino acid which may be of antioxidant activities，its content in MSCG-2 is 31. 3% higher than that in MSCG-1; (6) the oxygen radical absorbance capacities (ORAC) of MSCG-1 and MSCG-2 are (56. 10 ±18. 49) and (1077. 19 ± 60. 82) μmol/g; and (7) the antioxidant activity of MSCG-2 is significantly higher than those of MSCG and MSCG-1，which means that the molecular composition，molecular mass and struc-tural characteristics of glycoprotein determine their antioxidant activities．

Key words： Millettia Speciosa Champ．; glycoprotein; separation; purification; structure identification; antioxidants

Cite this article

ZHAO Qiang-Zhong , FENG Meng-Ying , LIN Lian-Zhu , ZHAO Mou-Ming . Separation，Identification and Antioxidant Activity of Glycoproteins from Millettia Speciosa Champ．[J]. Journal of South China University of Technology(Natural Science), 2015 , 43(11) : 8 -15 . DOI: 10.3969/j.issn.1000-565X.2015.11.002

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