Journal of South China University of Technology(Natural Science) >

2015 , Vol. 43 >Issue 2: 20 - 26

DOI: https://doi.org/10.3969/j.issn.1000-565X.2015.02.004

Food Science & Technology

Preparation of Frucooligosaccharides via Biotransformation by Aspergillus niger 6640

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  • School of Light Industry and Food Sciences，South China University of Technology，Guangzhou 510640，Guangdong，China
刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物利用与控制研究．

Received date: 2014-05-23

  Revised date: 2014-11-19

  Online published: 2014-12-31

Supported by

Supported by the National Natural Science Foundation of China(31101254)and the Natural Science Foundation of Guangdong Province (S2011010005679)

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Abstract

In this paper，the preparation conditions of fructooligosaccharides (FOS) via the biotransformation by Aspergillus niger 6640 (A．niger 6640) were optimized，and the properties of crude enzyme of β-Ffase from A． ni-ger 6640 were investigated． Then，β-Ffase was isolated and purified by using an anion column of DEAE Sepharose CL-6B，and its molecular mass was preliminarily measured by means of SDS-PAGE electrophoresis． The results show that (1) the optimal conditions for FOS preparation should be controlled at a pH value of 7． 6，a fermentation time of 40 h，a fermentation temperature of 33 ℃ and a sucrose mass fraction of 40%; (2) in the buffer of Na 2 HPO 4 -citric acid for the biotransformation using vacuum-dried β-Ffase，the optimal conditions are determined as pH 6． 5，a reaction time of 16h and a β-Ffase dosage of 0． 19g; and (3) the molecular mass of β-Ffase after isola-tion and purification is about 75ku．

Key words： Aspergillus niger 6640; fructooligosaccharides; β-fructofuranosidase; biotransformation

Cite this article

Liu Dong-mei Zhou Jin-song Shang Yong-he Yang Dan-xia Ke Yi-chun Yu Shu-juan . Preparation of Frucooligosaccharides via Biotransformation by Aspergillus niger 6640[J]. Journal of South China University of Technology(Natural Science), 2015 , 43(2) : 20 -26 . DOI: 10.3969/j.issn.1000-565X.2015.02.004

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