Journal of South China University of Technology (Natural Science Edition) ›› 2015, Vol. 43 ›› Issue (2): 20-26.doi: 10.3969/j.issn.1000-565X.2015.02.004

• Food Science & Technology • Previous Articles     Next Articles

Preparation of Frucooligosaccharides via Biotransformation by Aspergillus niger 6640

Liu Dong-mei Zhou Jin-song Shang Yong-he Yang Dan-xia Ke Yi-chun Yu Shu-juan   

  1. School of Light Industry and Food Sciences，South China University of Technology，Guangzhou 510640，Guangdong，China
  • Received:2014-05-23 Revised:2014-11-19 Online:2015-02-25 Published:2014-12-31
  • Contact: 刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物利用与控制研究． E-mail:liudm@scut.edu.cn
  • About author:刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物利用与控制研究．
  • Supported by:
    Supported by the National Natural Science Foundation of China(31101254)and the Natural Science Foundation of Guangdong Province (S2011010005679)

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Abstract: In this paper，the preparation conditions of fructooligosaccharides (FOS) via the biotransformation by Aspergillus niger 6640 (A．niger 6640) were optimized，and the properties of crude enzyme of β-Ffase from A． ni-ger 6640 were investigated． Then，β-Ffase was isolated and purified by using an anion column of DEAE Sepharose CL-6B，and its molecular mass was preliminarily measured by means of SDS-PAGE electrophoresis． The results show that (1) the optimal conditions for FOS preparation should be controlled at a pH value of 7． 6，a fermentation time of 40 h，a fermentation temperature of 33 ℃ and a sucrose mass fraction of 40%; (2) in the buffer of Na 2 HPO 4 -citric acid for the biotransformation using vacuum-dried β-Ffase，the optimal conditions are determined as pH 6． 5，a reaction time of 16h and a β-Ffase dosage of 0． 19g; and (3) the molecular mass of β-Ffase after isola-tion and purification is about 75ku．

Key words: Aspergillus niger 6640, fructooligosaccharides, β-fructofuranosidase, biotransformation