Journal of South China University of Technology (Natural Science Edition) ›› 2021, Vol. 49 ›› Issue (11): 9-18.doi: 10.12141/j.issn.1000-565X.210122

Special Issue: 2021年食品科学与技术

• Food Science & Technology • Previous Articles     Next Articles

Preparation and Anticoagulant Activity of Sulfated Schizophyllan Polysaccharides

ZHENG Bisheng1 WU Lei1 ZHOU Lin2 PEI Yunlin3   

  1. 1.School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China；2.School of Life Sciences and Biopharmaceuticals，Guangdong Pharmaceutical University，Guangzhou 511436，Guangdong，China；3.Guangdong Marumi Biotechnology Co.，Ltd.，Guangzhou 510530，Guangdong，China
  • Received:2021-03-10 Revised:2021-04-30 Online:2021-11-25 Published:2021-11-01
  • Contact: 郑必胜（1966-），男，博士，副教授，主要从事裂褶多糖的制备及改性研究。 E-mail:febzheng@scut.edu.cn
  • About author:郑必胜（1966-），男，博士，副教授，主要从事裂褶多糖的制备及改性研究。
  • Supported by:
    Supported by the Science and Technology Program of Guangdong Province, China（2020A050503017）

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Abstract: This paper mainly introduced the preparation and anticoagulant activity of sulfated Schizophyllan polysaccharides （S-SPG）.The single-factor and response surface method were used to optimize the preparation of polysaccharides-SPG with the sulfur trioxide-pyridine method.The anticoagulant activity was evaluated through APTT，TT，and PT assays.The results show that when the mass ratio of sulfur trioxide-pyridine complex to Schizophyllan polysaccharides （SPG） is 4，the reaction temperature is 70℃，and the reaction time is 3h，the degree of substitution of S-SPG was the highest （1.85）.The FT-IR and 13C NMR spectroscopy data proved that the sulfated group was successfully inserted into schizophyllan.Anticoagulant assays show that sulfated Schizophyllan polysaccharides exhibits strong anticoagulant activity and prolongs APTT and TT，but has no effect on PT.The anticoagulant acti-vity is positively correlated with the concentration and degree of substitution.S-SPG3 with the highest degree of substitution shows the strongest anticoagulant activity.When the mass concentration is 0.2g/L，the APTT values of S-SPG3 is 184.35s，which is 1.86 times of S-SPG2 and 2.49 times of S-SPG1 under the same concentration.Besides，the TT values of S-SPG3 was over 90 s at the mass concentration of 0.2g/L.

Key words: sulfated Schizophyllan polysaccharides, anticoagulant activity, sulfur trioxide-pyridine method, coagulation assays

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