Abstract: For the extraction of Sargassum fusiforme polysaccharide, the experimental factors and levels were selected through single factor experiment. According to the central combination experimental design principle of box benhnken, the response surface analysis method of three factors and three levels was adopted on the basis of single factor experiment, the influencing factors of various process conditions were determined according to regression analysis, and the response surface and contour map were made with the polysaccharide extraction rate as the response value. The results showed that, The optimum conditions of water extraction of Sargassum fusiforme polysaccharide were as follows: extraction temperature 85 ℃, extraction time 2.6h, water material ratio 28 ∶ 1; When extracted once, the extraction rate of Sargassum fusiforme polysaccharide reached 11.12%.

Key words: Sargassum fusiforme polysaccharide, extract, Response surface analysis