Journal of South China University of Technology (Natural Science Edition) ›› 2014, Vol. 42 ›› Issue (10): 1-6.doi: 10.3969/j.issn.1000-565X.2014.10.001

• Materials Science & Technology •     Next Articles

Cell Response to Calcium Phosphate Cement Incorporating Lyophilized Powders of PRP and PPP

Ye Jian-dong1,2  Wu Hua-e1,2  He Fu-po3  Zhang Jing1,2   

  1. 1. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; 2. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, Guangdong, China; 3. School of Basic Science, Guangzhou Medical University, Guangzhou 510182, Guangdong, China
  • Received:2014-04-01 Revised:2014-04-30 Online:2014-10-25 Published:2014-10-01
  • Contact: 叶建东（1959-），男，教授，博士生导师，主要从事生物医用材料研究. E-mail:jdye@scut.edu.cn
  • About author:叶建东（1959-），男，教授，博士生导师，主要从事生物医用材料研究.
  • Supported by:

    国家自然科学基金资助项目（51172074）

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Abstract:

Calcium phosphate cement (CPC) was modified by incorporating the lyophilized powders of plateletrich plasma (PRP) and plateletpoor plasma (PPP), and the effects of PRP or PPP on the hydration product phase, section morphology and cell response of CPC were characterized by means of XRD, SEM and cell experiments. The results show that (1) incorporating PRP or PPP does not affect the hydration products of CPC, and the final hydration product is nothing but poorly crystalline hydroxyapatite; (2) incorporating an appropriate amount of lyophilized powders of PRP or PPP into CPC greatly promotes the adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs) on CPC; and (3) in the late stage of cell culture, incorporating a smaller amount of PRP helps to improve cell proliferation and differentiation. Therefore, it is expected that PRP and PPP can be used to improve the cell response and osteoinductivity of CPC.

Key words: platelet-rich plasma, platelet-poor plasma, calcium phosphate cement, cell response, osteoinductivity