Journal of South China University of Technology (Natural Science Edition) ›› 2005, Vol. 33 ›› Issue (11): 51-54.

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Construction，Screening and Identification of Phage scFv Library Against Clenbuterol

Pan Ke1  Wang Hong1  Zhang Hong-bin2  Wang Jie2  Lei Hong-tao1  Yang Jin-yi1  Sun Yuan-ruin1   

  1. 1.The Key Laboratory of Food Quality and Safety,Dept.of Education of Guangdong Province,South China Agricultural Univ Guangzhou 510642,Guangdong,China;2.Center of Medical Research,General Hospital of Guangzhou Military Command,Guangzhou 510010,Guangdong,China
  • Received:2005-09-21 Online:2005-11-25 Published:2005-11-25
  • Contact: 王弘，E-mail：gzwhongd@163．com
  • About author:潘科(1977-)，男，博士生，主要从事食品安全快速检测研究
  • Supported by:

    国家自然科学基金资助项目(30400354，20477012)；广东省自然科学基金资助项目(04300502)

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Abstract:

Phage single·chain Fv(scFv)libraries with a size of about 1.6×1 0 were constructed for clenbuterol (CBL)by the phage display technology,and then,specific anti.CBL scFv was screened.Thus,the variable heavy (VH)and light(VL)anti·CBL genes were successfully amplified and scFv gene was assembled by overlapping PCR.After 4 times of enriched procedure in the order of adsorption- elution- amplification , 6 CBL-positive phage scFv clones were screened by ELISA.This research lays a foundation for the further expression of anti CBL scFv.

Key words: clenbuterol, single-chain Fv, phage display