Journal of South China University of Technology(Natural Science Edition) ›› 2019, Vol. 47 ›› Issue (6): 142-148.doi: 10.12141/j.issn.1000-565X.180454

• Biotechnology • Previous Articles    

Comparisons of Humanized AGR2 Monoclonal Antibodies Expressions in Different Host Cells

XIE Qiuling1,2 TANG Jie1 HUANG Jiahui3 LU Jia2 LIU Chenxuexuan1   

  1. 1． College of Life Science and Technology，Jinan University，Guangzhou 510632，Guangdong，China; 2． National Engineering Research Center of Genetic Medicine，Guangzhou 510000，Guangdong，China; 3． College of Yingdong Life Science，Shaoguan University，Shaoguan 512005，Guangdong，China 
  • Received:2018-09-10 Revised:2018-12-07 Online:2019-06-25 Published:2019-05-05
  • Contact: 谢秋玲(1968-)，女，博士，研究员，主要从事重组抗体表达等研究． E-mail:txql@jnu.edu.cn
  • About author:谢秋玲(1968-)，女，博士，研究员，主要从事重组抗体表达等研究．
  • Supported by:
    Supported by the Science and Technology Major Project of Guangdong Province(2012A080202014) and Science and Technology Planning Project of Guangdong Province(2015A020211016) 

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Abstract: Anterior gradient-2 (AGR2) gene is expressed in many organs of human body，which is closely related to tumor growth，metastasis，invasion and drug resistance． In this study，we successfully developed a humanized monoclonal antibody，rhAGR2-mAb，which can specifically binds with AGR2 to be a new anti-tumor drug． In or- der to evaluate the activity of this antibody，rhAGR2-mAb was expressed with HEK293F cells and CHO cells by transient transfections and this protein with 95% purity was obtained． Then the properties of rhAGR2-mAb inclu- ding molecular weights，isoelectric points，glycan structures expressed in CHO cells and HEK cells were com- pared． The results show that there are no detected differences between HEK293F cells and CHO cells on molecular weights，isoelectric points，glycosylation types． However，the product of two expressions are found different in glycosylation types proportion，which makes them differ in affinities with AGR2． This provides data for selecting a suitable expression system to transiently and stably express rhAGR2-mAb in the future．

Key words: humanization, AGR2 monoclonal antibody, transient expression, CHO cell, HEK cell

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