Journal of South China University of Technology (Natural Science Edition) ›› 2019, Vol. 47 ›› Issue (11): 62-70.doi: 10.12141/j.issn.1000-565X.190101

• Biotechnology • Previous Articles     Next Articles

Asymmetric Oxidation of Ｒacemic 1-Phenyl-1，2-Ethanediol Using Immobilized Kurthia gibsonii SC0312 Cells in DESs-Containing System

LOU Wenyong ZHAO Ying PENG Fei   

  1. School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China
  • Received:2019-03-19 Revised:2019-04-10 Online:2019-11-25 Published:2019-10-02
  • Contact: 娄文勇(1975-)，男，教授，博士生导师，主要从事生物催化研究． E-mail:wylou@scut.edu.cn
  • About author:娄文勇(1975-)，男，教授，博士生导师，主要从事生物催化研究．
  • Supported by:
    Supported by the National Natural Science Foundation of China( 21676104，21878105)

PDF

57

Abstract: Highly efficient asymmetric oxidation of racemic 1-phenyl-1，2-ethanediol ( PED) was successfully con- ducted to prepare enantiomerically pure ( S) -PED with immobilized Kurthia gibsonii SC0312 cells in non-aqueous system containing DESs． Among the 4 kind of tested ionic liquids and 2 kind of deep eutectic solvents，［ChCl］［U］ manifested better biocompatibility with Kurthia gibsonii SC0312 cells，and it can appropriately change the cell membrane integrity，thereby effectively improve the catalytic efficiency of this reaction． When ［ChCl］［U］content is 4% ( volume fraction) ，buffer pH is 7． 5，reaction temperature is 35 ℃，cell concentration is 60 mg /mL，and substrate concentration is 100 mmol /L，the initial reaction rate reaches 8． 25 mmol /( L·h) ，and after 7 hours' re- action，the yield rate and ( S) -PED e． e． value reach 49． 6% and 99． 9% ，respectively．

Key words: Kurthia gibsonii SC0312 cells, 1-phenyl-1, 2-ethanediol, asymmetric oxidation, ［ChCl］［U］

CLC Number: