Journal of South China University of Technology(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (12): 122-127.

• Biological Engineering • Previous Articles     Next Articles

Expression of Green Fluorescent Protein-Tagged S2P Homologs in Synechocystis sp. PCC 6803

Chen Gu  Wen Pan-pan  Qin Chun-yan  Wang Yu-ling   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2012-03-31 Revised:2012-08-02 Online:2012-12-25 Published:2012-11-02
  • Contact: 陈谷(1973-) ，女，博士，副教授，主要从事分子生物学研究． E-mail:chengu@scut.edu.cn
  • About author:陈谷(1973-) ，女，博士，副教授，主要从事分子生物学研究．
  • Supported by:

    国家自然科学基金资助项目( 30800609， 31270085) ; 华南理工大学中央高校基本科研业务费专项资金资助项目( 2009ZM0006)

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Abstract:

In order to explore the functions of site-2 protease ( S2P) homologs Slr0643 and Sll0862 in Synechocystis sp. PCC 6803,enhanced green fluorescent protein ( EGFP) was added to the carboxyl terminal as a protein tag,and two transgenic Synechocystis sp. PCC 6803,namely,psbA2: : 0643GFP-Cmr /△0643-Kmr and psbA2: : 0862GFP-Cmr /△0862-Kmr,were obtained. The transcriptional expression of egfp in two transgenic lines was then detected via RT-PCR,and the GFP fluorescence was observed by using a laser scanning confocal microscope. Both of these confirm the successful expression of S2P fusion proteins tagged with EGFP under the psbA2 promoter.

Key words: enhanced green fluorescent protein, site-2 protease homolog, Synechocystis sp. PCC 6803, fusion protein

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