Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (5): 133-138.doi: 10.3969/j.issn.1000-565X.2010.05.026

• Biological Engineering • Previous Articles     Next Articles

Investigation into Novel Nanocomposites as Non-Viral Gene Carrier

Wang Xiao-ying1  Liu Bo1  Lin Lu1  Wu Jun2  Sun Run-cang1   

  1. 1.State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;2.South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,Guangdong,China
  • Received:2009-06-09 Revised:2009-09-27 Online:2010-05-25 Published:2010-05-25
  • Contact: 王小英（1978-），女，博士，讲师，主要从事再生资源化学生物学研究． E-mail:xyw@seut．edu．cn
  • About author:王小英（1978-），女，博士，讲师，主要从事再生资源化学生物学研究．
  • Supported by:

    国家“973”计划项目（2010CB732201）; 国家自然科学基金资助项目（30972323 30710103906）; 中国科学院海洋生物资源可持续利用重点实验室、广东省应用生物学重点实验室和海洋药物重点实验室联合开放基金资助项目（LMB081002）

PDF

807

Abstract:

In order to obtain ideal non-viral gene carriers,trisaccharide-g-chitosan（TCS） was synthesized and was intercalated into rectorite（REC） to prepare novel nanocomposite.The product was then characterized by means of XRD and TEM,with its cellular compatibility being also analyzed.Moreover,REC,TCS and the prepared nanocomposite were respectively combined with plasmid DNA to form novel complexes,whose gene transfection effects in vitro were also evaluated.The results indicate that REC,TCS and the prepared nanocomposite all have good cellular compatibility,that the TCS/DNA complex is about 75nm in size at a N/P ratio of 3,that the addition of REC may increase the size up to 191nm,that the stability of TCS/DNA complex is positively correlated to the relative molecular mass of TCS and may degrade due to the addition of REC,and that TCS/REC-DNA complex can enter into hepatoma cells with fluorescence expression.It is thus concluded that TCS/REC nanocomposite is a potential non-viral gene carrier.

Key words: trisaccharide-g-chitosan, rectorite, intercalation, nanocomposite, gene transfection