Journal of South China University of Technology (Natural Science Edition) ›› 2009, Vol. 37 ›› Issue (12): 90-94.

• Biological Engineering • Previous Articles     Next Articles

Enzymatic Synthesis of 2-Chloro-1-Phenylethanol with High Optical Purity

Xia Xiao-le1  Wang Yong-hua2  Li Wen-cheng1  Yang Bo1  Wang Xiao-ning1   

  1. 1. School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China; 2. School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China
  • Received:2008-10-07 Revised:2009-07-03 Online:2009-12-25 Published:2009-12-25
  • Contact: 夏小乐（1980-），男，博士生，主要从事生物催化和生物计算研究． E-mail:xiaxiaole@210n．com
  • About author:夏小乐（1980-），男，博士生，主要从事生物催化和生物计算研究．
  • Supported by:

    国家自然科学基金资助项目（20506007,20706021）

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Abstract:

Two enantiomers of chiral 2-chloro-1-phenylethanol with high optical purity were synthesized using the enzymatic method, and the kinetic resolution of various lipases were investigated. Moreover, the effects of organic solvent, temperature and water activity on the lipase-catalyzed resolution of （R, S）-2-chloro-1-phenylethanol were analyzed, and the reuse of immobilized enzymes was investigated. The results indicate that a new immobilized lipase from Pseudomonas cepacia marked as Lipase PS IM shows high activity, by which two high-purity enantiomers （eep 99% and ees 99% , respectively） can be separated in n-hexane at 35℃with a water activity of 0. 69; and that the relative activity of Lipase PS IM after five repetitive reactions is still up to 85%.

Key words: 2-chloro-1-phenylethanol, kinetic resolution, water activity, lipase, wheat germ lipase