Journal of South China University of Technology (Natural Science Edition) ›› 2008, Vol. 36 ›› Issue (3): 64-68.

• Chemistry & Chemical Engineering • Previous Articles     Next Articles

Extraction Optimization and Component Identification of Tobacco Polyphenols

Wang Hai-yan1  Cui Chun1  Zhao Mou-ming1  Shen Guang-lin2  Rao Cuo-hua2   

  1. 1. School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong,China; 2. Technology Center of Guangdong Cigarette General Factory, Guangzhou 510515, Guangdong, China
  • Received:2007-07-09 Revised:2007-09-24 Online:2008-03-25 Published:2008-03-25
  • Contact: 赵谋明，教授，博士生导师。 E-mail:femmzhao@scut.edu.cn
  • About author:王海燕（1970-），女，博士生，中国烟草总公司职工技术培训中心讲师，主要从事烟草化学研究。E-mail：tobacco0710@sohu.com
  • Supported by:

    国家十一五科技支撑计划项目（2006BAD27803）

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Abstract:

In this paper, tobacco polyphenols were extracted through microwave-assisted technology, and their main components were identified by the combination of hgh-performance liquid chromatography, photodiode array detector and mass spectrography. Moreover, the effects of microwave power, methanol content, extraction time and material-to-liquid ratio on the polyphenols yield were investigated. It is seen from the response surface analysis （RSA） that, in the optimum extraction conditions for tobacco polyphenols, that is, With 53 % methanol as the solvent, a material-to-solvent ratio of 1：11, a microwave power of 560 W and an extraction time of 70 s, the yield of total to- bacco polyphenols reaches 22.38 mg/g （dry weight）. HPLC-PDA-MS results demonstrate that the main components in tobacco polyphenols are chlorogenic acid and rutin.

Key words: tobacco, polyphenol, microwave extraction, response surface analysis