Journal of South China University of Technology (Natural Science Edition) ›› 2008, Vol. 36 ›› Issue (12): 97-101.

• Biological Engineering • Previous Articles     Next Articles

Improvement of Lipid Content of Chlorella pyrenoidosa Two-Step Cultivation

Huang Guan-hua  Chen Feng  Wei Dong   

  1. School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China
  • Received:2008-03-28 Revised:1900-01-01 Online:2008-12-25 Published:2008-12-25
  • Contact: 陈峰，教授、博士生导师． E-mail:sfchen@hku．hk
  • About author:黄冠华（1981-），女，博士生，主要从事微藻生理及其应用研究．E—mail：huangghcLara@yahoo．com．cn
  • Supported by:

    国家自然科学基金-广东省自然科学基金联合基金重点项目（u0633009）;国家科技部“十一五”科技支撑计划项目（2006BAD27B03）;广东省科技攻关项目（20052050166）;广州市科技计划项目（2005Z3-E0331）

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Abstract:

Based on the growth rules of Chlorella pyrenoidosa （ C. pyrenoidosa） and the characteristics of intracellular lipid accumulation in nitrogen-deficient condition, the improvement of lipid content of C. pyrenoidosa using a two-step cultivation method was investigated. The results show that （ 1 ） the optimal mass concentrations of glucose and KNO3 for the high-density cultivation of C. pyrenoidosa are respectively 20 and 1. 50 g/L; （2） with 1.50 g/L KNO3 as the nitrogen source, the biomass of C. pyrenoidosa increases from （7.55 ± 0. 23 ） g/L in the single-step cultivation to （9. 23 ±0. 11 ） g/L in the two-step cultivation; （3） the contents of intracellular lipid and total fatty acids in the two-step cultivation .respectively increase by 5.8% and 5.2% ; and （4） the calorific values improves from （23.58 ±0. 02） kJ/g to （25.52±0.03） kJ/g. It is thus concluded that the two-step cultivation not only results in a high-densitv cultivation of C. pyrenoidosa but also effectively increases the cellular linid content.

Key words: Chlorella pyrenoidosa, lipid, nitrogen deficiency, calorific value, two-step cultivation