Journal of South China University of Technology (Natural Science Edition) ›› 2008, Vol. 36 ›› Issue (12): 128-132.

• Biological Engineering • Previous Articles     Next Articles

Application of ISSR Marker and ITS Sequence to Investigation of Genetic Variation of Aquilaria sinensis

Shen Yan-jing1  Yan Ping1  Zhao Xuan1  Pang Qi-hua1  Zhao Shu-jin2     

  1. 1. School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China; 2. Department of Medicament, General Hospital of G uangzhou Military Command, Guangzhou 510010, Guangdong, China
  • Received:2007-09-18 Revised:2008-03-06 Online:2008-12-25 Published:2008-12-25
  • Contact: 申彦晶（1980-），女，博士生，主要从事生物制药研究． E-mail:yanjingshen2004@163．com
  • About author:申彦晶（1980-），女，博士生，主要从事生物制药研究．
  • Supported by:

    广东省自然科学基金资助项目（06019716）

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Abstract:

This paper deals with the genetic variation, genetic relationship and molecular identification of different populations of Aquilaria sinensis （A. sinensis） by employing the inter-simple sequence repeat （ISSR） marker and the internal transcribed spacer （ITS） sequence of ribosomal RNA （rDNA）. The results of ISSR marker show that 60. 0% of the 80 bands amplified with 7 ISSR primers are polymorphic bands, and that the genetic distance among the 9 populations of A. sinensis varies from 0. 073 3 to 0. 4213. The results of rDNA ITS sequence show that there are only 6 variable sites in the 9 populations, with a genetic distance varying from 0. 0000 to 0. 0110. Moreover, as the cluster graphs respectively obtained from the ISSR marker and the ITS sequence, different in shape but similar in trend, well describe A. sinensis and its related species in different populations, both the ISSR marker and the ITS sequence are effective in revealing the genetic variation and genetic relationship of A. sinensis.

Key words: Aquilaria sinensis, inter-simple sequence repeat, internal transcribed spacer, genetic variation