Journal of South China University of Technology (Natural Science Edition) ›› 2007, Vol. 35 ›› Issue (2): 89-92.

• Materials Science & Technology • Previous Articles     Next Articles

Preparation and Properties of the Sensing Membranes Immobilizing Plant Esterase-Chromogenic Agent

Ma Wen-shi1  wang Jun1  Luan Chong-lin2   

  1. 1. School of Materials Science and Engineering , South China Univ. of Tech. , Guangzhou 510640 , Guangdong , China;2. Dept. of Fine Chemistry , Shenzhen Polytechnic , Shenzhen 518055 , Guangdong , China
  • Received:2005-12-01 Online:2007-02-25 Published:2007-02-25
  • Contact: 马文石(1962-)，男，博士，副教授，主要从事功能材料、胶粘剂研究. E-mail:mcwshma@scut. edu.cn
  • About author:马文石(1962-)，男，博士，副教授，主要从事功能材料、胶粘剂研究.

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Abstract:

PV AlSi02 organic-inorganic hybrid sol was prepared by means of in-situ sol-ge1 method , with poly( vinyl alcohol) (PV A) and tetraethylorthosilicate (TEOS) as the materials. Then , the sol with the dispersion of chromo-genic agent and plant esterase was dried to form a kind of transparent sensing membrane with controllable hydrophi-licity-acetonephilicity and good storability. It is shown that , (1) when the membrane is soaked in α-naphthyl ace-tate solution , an absorption band occurs at 520 nm in the ultravio1et-visible spectrum; (2) the enzyme activity of the sensing membrane obviously decreases in 12 days until it reaches a constant value; (3) the decreasing rate of enzyme activity of the sensing membrane in cold storage is significantly smaller than that in room temperature storage; and (4) the stability of the enzyme immobilized in the hybrid membrane is greatly improved , as compared with that of the enzyme in solution.

Key words: poly (vinyl alcohol), silica, organic-inorganic hybrid, plant esterase, chromogenic agent, sensing membrane