Journal of South China University of Technology (Natural Science Edition) ›› 2007, Vol. 35 ›› Issue (12): 66-70,96.

• Materials Science & Technology • Previous Articles     Next Articles

Influence of EDC/NHS Crosslinking on Physicochemical Properties of Collagen

Wang Ying-jun  Yang Chun-rong  Wang Ling-yun   

  1. School of Materials Science and Engineering , South China Univ. of Tech. , Guangzhou 510640 , Guangdong , China
  • Online:2007-12-25 Published:2007-12-25
  • Contact: 王迎军(1954-) ，女，教授，博士生导师，主要从事生物医用材料和组织工程材料研究. E-mail:imwangyj@scut. edu. cn
  • About author:王迎军(1954-) ，女，教授，博士生导师，主要从事生物医用材料和组织工程材料研究.
  • Supported by:

    国家"973" 重大基础研究项目(2006CB623知) ;广东省自然科学基金团队项目(4205786 )

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Abstract:

In order to improve the physicochemical properties of collagen , l-ethyl-3 -( 3 -dimethyl aminopropyl) carbodiimide (EDC) /N -hydroxysuccinimide (NHS) was used as a crosslinking agent for collagen matrices , and the effect of crosslinking agent dosage on the physicochemical properties of collagen was investigated. Then , the measurements of FT -IR , DSC and SEM were performed and the water absorption , the swelling ratio and the collagenase resistance were tested to characterize the physicochemical properties of the collagen before and after the crosslinking. The results demonstrate that , after the crosslinking , the thermal stability , the morphological stability and the enzymatic resistance all improve significantly , and that the disordered microstructure of collagen changes to a dense and ordered state. It is thus concluded that the EDC/NHS crosslinking greatly improves the physicochemical properties of collagen.

Key words: collagen, crosslinking, physicochemical property, l-ethyl-3- (3 -dimethyl aminopropyl) carbodiimide, N -hydroxysuccinimide