Journal of South China University of Technology (Natural Science Edition) ›› 2006, Vol. 34 ›› Issue (5): 58-62.

• Biology Science • Previous Articles     Next Articles

Efect of Augmenter of Liver Regeneration on Na+，K+-ATPase Activity of HepG2 Cell

Lin Ying1  Tong Ming-hua2  Kong Xiang-ping2  Liang Shi-zhong1   

  1. 1.College of Biological Science and Engineering,South China Univ.of Tech.,Guangzhou 5 10640,Guangdong,China;2.Liver Diseases Research Institute.Guangzhou Hospital of Air-Force,Guangzhou 510602,Guangdong,China
  • Received:2005-07-07 Online:2006-05-25 Published:2006-05-25
  • Contact: 孔祥平，主任医师，E-mail：xiangpmg_kong@hntmail.com E-mail:ly9a9@126.com
  • About author:林莹(1971-)，女，博上牛，主要从事微生物发酵和生物制药研究．
  • Supported by:

    广东省重点科技项目基金(99B04704G-47-1)

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Abstract:

The effect of recombinant augmenter of liver regeneration(ALR)on the proliferation of HepG2 cell was investigated by the MTY(Thiazolyl blue)assay,and the activity of the Na ,K -ATPase was measured by an in-organic phosphorus spectrophotometer.Then,the phosphorylation of Na+,K+-ATPase was discussed by means of protein immunoblot.The results show that recombinant ALR promotes the proliferation of HepG2 cell in vitro.As for the mechanism.the stimulation of Na+,K+-ATPase in HepG2 cell caused by ALR accords with the Michaelis-Menten equation model.Moreover,ALR improves the turnover rate of Na+,K+-ATPase-the value of Vmax increases from(0.84±0.11)μmol/(mg·min)to(1.68±0.07)μmol/(mg·min)(P<0.01)and that of Km decreases from(23.54±0.12)mmol/L to(20.86±0.13)mmol/L(P>0.05).Also,the resuhs demonstrate that the phosphorylation of serine or threonine is key to the stimulation of Na+,K+-ATPase.A dose-time dependence of the phosphorylation of Na+,K+-ATPase in HepG2 cell stimulated by ALR is finally revealed by means of protein im-munoblot.

Key words: liver regeneration, augmenter, Na+,K+-ATPase , turnover rate, dose—time dependence