Journal of South China University of Technology (Natural Science Edition) ›› 2006, Vol. 34 ›› Issue (12): 41-46.

• Biological Engineering • Previous Articles     Next Articles

Enzymatic Preparation of ACEIP Based on Structure Information

Zhu Zhi-wei1  Tu Xiao-ke2  Zeng Qing-xiao1  Ruan Zheng1  Zhang De-cong3   

  1. 1.School ofLight Industry and Food Sciences,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China;2.Food Inspoetion and Quarantine Center,Shenzhen Entry·Exit Inspection and Quarantine Bureau,Shenzhen 518067,Guangdong,China;3.Schol of Chemical Science,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China
  • Received:2005-10-20 Online:2006-12-25 Published:2006-12-25
  • Contact: 朱志伟(1974-), 男, 博士, 讲师, 主要从事食品科学与工程研究. E-mail:zhwzhu@ scut. edu. cn
  • About author:朱志伟(1974-), 男, 博士, 讲师, 主要从事食品科学与工程研究.
  • Supported by:

    广东省自然科学基金博士启动基金资助项目( 04300090); 华南理工大学自然科学基金青年基金资助项目( E5040970)

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Abstract:

The aim of this research is to investigate the enzymatic preparation of angiotensin Ⅰ-converting enzyme in-hibitory peptide(ACEIP)on the basis of the structure information analysis and by choosing enzymes tII preferen-tial specificity,thus preliminarily giving a directional and controlled enzymolysis technology according to the struc-ture information．In the investigation,the structure information of 1 1 selected angiotensin Ⅰ-converting enzyme in-hibitors was analyzed by means of the Complete Neglect of Diferential Overlap(CNDO).The results indicate that the oligopeptide possesses high inhibitory activity to angiotensin I-converting enzyme(ACE)when it is of residues Pro,Trp,Met,Phe and Lys in C·term inal position an d when residues Asp an d Glu in N-terminal position.Th en,the enzymatic preparation of ACEIP from tilapia meat was conducted using enzymes with preferential specificity.Th e hydrolysate,in which Thr-Cys,Asp-Trp an d Glu·Met were found,was finally extracted and analyzed.The re-suits accord with the theoretical results,which preliminarily shows the feasibility of the directional and controlled enzymatic technology based on the structure inform ation.

Key words: angiotensin Ⅰ-converting enzyme inhibitory peptide, enzymolysis, structure inform ation, Complete Neglect of Diferential Overlap(CNDO)