Abstract: Agrobacterium rhizogenes R1601was used to induce the hairy roots from cotyledons and stems of Trichosanthes bracteata Voigt‚The cultivation system for the growth of hairy roots was established in a photobioreactor． The effects of light intensity‚dissolved-oxygen saturation and carbon source on the growth of hairy roots were then investigated．The results show that the specific growth rate of hairy roots maintains higher level when the dissolved-oxygen saturation and light intensity are respectively controlled at 80％ and 25 W／m 2．It is also shown that sucrose sources affect remarkably growth of hairy roots than glucose．When the sucrose concentration is controlled at30～40 g／L‚the biomass in the two grades culture system（9．5g／L） is20％ more than biomass of hairy roots in the single culture system（7．2g／L）．

Key words: Trichosanthes bracteata Voigt, hairy root, Agrobacterium rhizogenes, photobioreactor, growth, cultivation