Journal of South China University of Technology (Natural Science Edition) ›› 2015, Vol. 43 ›› Issue (6): 135-141.doi: 10.3969/j.issn.1000-565X.2015.06.021

• Biological Engineering • Previous Articles     Next Articles

Identification and Nitrite Degradation Ability of Bacillus cereus LJ01

Liu Dong-mei Luo Tong-hui Yang Dan-xia Huang Juan Wu Hui Yu Yi-gang Li Li   

  1. School of Light Industry and Food Sciences，South China University of Technology，Guangzhou 510640，Guangdong，China
  • Received:2014-10-28 Revised:2015-01-17 Online:2015-06-25 Published:2015-05-04
  • Contact: 刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物的利用与控制研究． E-mail:liudm@scut.edu.cn
  • About author:刘冬梅(1972-)，女，博士，副教授，主要从事食品微生物的利用与控制研究．
  • Supported by:
    Supported by the National Natural Science Foundation of China(31101254)，the Natural Science Foundation of Guangdong Province (S2011010005679) and the Key Science and Technology Program of Guangdong Province(2013B020312002，2014A020208019，412051029089)

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Abstract: A strain LJ01 with strong nitrite degradation ability was isolated from fermented bean paste and was iden-tified as Bacillus cereus． Then，the primary cell localization of this nitrite reductase was examined by measuring the enzyme activity of different cellular components from LJ01 cell． After LJ01 was induced by 100 mg/L sodium nitrite solution and treated with lysozyme，the crude enzyme solution of LJ01 was first separated by means of the anion DEAE Sepharose Fast Flow chromatography，and the nitrite degradation activity of fractions a，b and c was measured． Moreover，by using inorganic electron donors such as 0. 1mol/L succinic acid and sodium sulfite solu-tion，fraction c was identified as a critical nitrite reductase and was separated by Sephadex G-150 column to get pure protein． The identification results show that 0. 54mg of active enzyme protein with an activity of 4004. 89U/mg can be obtained from 1L of the fermentation liquid，and that the specific NiR activity of the purified enzyme increa-ses by 17. 57 folds with a recovery of 2. 37%． In addition，SDS-PAGE results show that the monomer molecular mass of LJ01 is about 30ku．

Key words: Bacillus cereus, nitrite reductase, nitrite degradation, 16S rDNA, cell localization

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