Journal of South China University of Technology (Natural Science Edition) ›› 2013, Vol. 41 ›› Issue (1): 127-132.doi: 10.3969/j.issn.1000-565X.2013.01.021

• Food Science & Technology • Previous Articles     Next Articles

Induction Conditions and EMA-qPCR Detection of Staphyloccocus aureusin Viable but Non-Culturable State

Yu Yi-gang Tian Cong Xiao Xing-long Tao Wen-yang Li jin-xia Wu Hui   

  1. School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China
  • Received:2012-05-25 Revised:2012-08-10 Online:2013-01-25 Published:2012-12-03
  • Contact: 肖性龙(1977-),男,博士,讲师,主要从事食品安全与检测研究. E-mail:fexxl@scut.edu.cn
  • About author:余以刚(1968-),男,博士,副教授,主要从事食品安全与检测研究.E-mail:yuyigang@scut.edu.cn
  • Supported by:

    国家自然科学基金青年科学基金资助项目(31101279);教育部高等学校博士学科点专项科研基金新教师类资助课题(20110172120034);华南理工大学中央高校基本科研业务费专项资金资助项目(2011ZM0101);广东省天然产物绿色加工与产品安全重点实验室开放课题(201101)

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Abstract:

In order to establish a model for the determination of induction conditions of Staphyloccocus aureus in via-ble but non-culturable (VBNC) state and to reveal the effects of temperature, salt concentration and pH value onthe culturable cell number of Staphyloccocus aureus, orthogonal experiments were carried out to optimize the inductionconditions. Then, the culturable cells were monitored and a method based on the DNA-binding dye EMA (EthidiumBromide Monoazide) and the qPCR technology was proposed to detect Staphyloccocus aureus in VBNC state. Theresults show that pH value greatly influences the culturable cell number, and that the cultivation in a nutrient brothmedium with 15% (mass fraction) NaCl and 0.3% (volume fraction) acetic acid at 4℃for 12d is best for theinduction of Staphyloccocus aureus in VBNC state. Moreover, it is indicated that EMA-qPCR is an effective methodto detect Staphyloccocus aureus in VBNC state under different induction conditions, with a Ct value improvement(cycle threshold) varying from 1.29 to 8.56.

Key words: Staphyloccocus aureus, viable but non-culturable state, induction condition, ethidium bromide monoa-zide, qPCR