Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (12): 129-134.doi: 10.3969/j.issn.1000-565X.2010.12.025

• Biological Engineering • Previous Articles     Next Articles

Mechanism of Enzyme Activity Variation of Catalase with Ultrasonic Treatment

Li Bing  Wang Wen-zong  Lin Hong-jia  Chen Ling  Li Lin   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2010-05-12 Revised:2010-06-19 Online:2010-12-25 Published:2010-12-25
  • Contact: 李琳（1963-），男，博士，教授，主要从事糖类物质及其药物的制备与生物利用研究． E-mail:felinli@scut．edu．cn
  • About author:李冰（1972-），女，博士，副教授，主要从事糖类物质及其药物的制备与生物利用研究．E-mail：bli@scut．edu．cn
  • Supported by:

    国家“863”计划重点项目（2007AA100405）

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Abstract:

In order to reveal the influencing mechanism of ultrasonic on the antioxidation defense system of microorganisms,catalase（CAT） was treated with ultrasonic and the effects of ultrasonic parameters on the enzyme activity of CAT were studied.Moreover,the secondary structure of CAT with and without ultrasonic treatment were analyzed by means of FT-IR.The results show that（1） the enzyme activity of CAT decreases with the increase in ultrasonic power;（2）the prolonging of irradiation time has no obvious effect on the enzyme activity of CAT;（3） the stable temperature and pH value for CAT treated with ultrasonic are respectively 37℃ and 7.4;and（4） after ultrasonic treatment,the kinetic parameters Km and Vmax of CAT both increase,and the secondary structure transfers from an ordered structure to a disordered one.

Key words: catalase, ultrasonic, enzyme activity, secondary structure