Journal of South China University of Technology(Natural Science Edition) ›› 2025, Vol. 53 ›› Issue (4): 125-134.doi: 10.12141/j.issn.1000-565X.240156

• Food Science & Technology • Previous Articles     Next Articles

Study on the Separation of Borneol Essential Oil and the Effects of Its Different Components on the Motility and Biofilm Formation of Escherichia coli

FAN Penghui1,2　CHEN Guanghao1　LIU Zirui1　CAI Xinyu1　WAN Yuanyuan1　SU Jianyu1,2   

  1. 1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China;

    2. Guangzhou Ruile Technology Co., Ltd., Guangzhou 510555, Guangdong, China

  • Online:2025-04-25 Published:2024-08-30

Abstract:

In order to explore the effect of different fractions of borneol essential oil on the motility and biofilm formation of Escherichia coli, Gas Chromatography-Mass Spectrometry (GC-MS) was used to analyze the volatile components of borneol essential oil crude, light fraction, and heavy fraction. The impact of these three components on the biofilm formation of Escherichia coli ATCC 25922 was investigated. The research results showed that the crude borneol essential oil, light fraction, and heavy fraction had 27, 24, and 19 volatile components, respectively. The volatile components with the highest relative content were (-)-borneol (19.93%), cineole (19.36%), and (-)-borneol (28.92%), respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the three components against Escherichia coli were all 6.25 μL·mL-1, and they could delay bacterial growth at sub-inhibitory concentrations. At 1/2 MIC and 1/4 MIC concentrations, all three borneol essential oil fractions could reduce the swimming and swarming abilities of Escherichia coli and inhibit the biofilm growth, with the heavy fraction showing the strongest inhibitory effect. In conclusion, molecular distillation can enrich the antibacterial active components in borneol essential oil, effectively enhancing its added value, and has broad application prospects.

Key words: borneol essential oil, molecular distillation; Escherichia coli, biofilm